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dc.creatorGaffield, Michael A.es
dc.creatorTabares, Lucíaes
dc.creatorBetz, William J.es
dc.date.accessioned2023-05-22T12:53:37Z
dc.date.available2023-05-22T12:53:37Z
dc.date.issued2009-03
dc.identifier.citationGaffield, M.A., Tabares, L. y Betz, W.J. (2009). The spatial pattern of exocytosis and post-exocytic mobility of synaptopHluorin in mouse motor nerve terminals. JOURNAL OF PHYSIOLOGY-LONDON, 587 (6), 1187-1200. https://doi.org/10.1113/jphysiol.2008.166728.
dc.identifier.issn0022-3751es
dc.identifier.urihttps://hdl.handle.net/11441/146493
dc.description.abstractWe monitored the spatial distribution of exo- and endocytosis at 37◦C in mouse motor nerve terminals expressing synaptopHluorin (spH), confirming and extending earlier work at room temperature, which had revealed fluorescent ‘hot spots’ appearing in repeatable locations during tetanic stimulation. We also tested whether hot spots appeared during mild stimulation. Averaged responses from single shocks showed a clear fluorescence jump, but revealed no sign of hot spots; instead, fluorescence rose uniformly across the terminal. Only after 5–25 stimuli given at high frequency did hot spots appear, suggesting a novel initiation mechanism. Experiments showed that about half of the surface spH molecules were mobile, and that spH movement occurred out of hot spots, demonstrating their origin as exocytic sources, not endocytic sinks. Taken together, our results suggest that synaptic vesicles exocytose equally throughout the terminal with mild stimulation, but preferentially exocytose at specific, repeatable locations during tetanic stimulation.es
dc.formatapplication/pdfes
dc.format.extent14 p.es
dc.language.isoenges
dc.publisherWileyes
dc.relation.ispartofJOURNAL OF PHYSIOLOGY-LONDON, 587 (6), 1187-1200.
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 Estados Unidos de América*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/us/*
dc.titleThe spatial pattern of exocytosis and post-exocytic mobility of synaptopHluorin in mouse motor nerve terminalses
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Fisiología Médica y Biofísicaes
dc.relation.publisherversionhttps://physoc.onlinelibrary.wiley.com/doi/full/10.1113/jphysiol.2008.166728es
dc.identifier.doi10.1113/jphysiol.2008.166728es
dc.journaltitleJOURNAL OF PHYSIOLOGY-LONDONes
dc.publication.volumen587es
dc.publication.issue6es
dc.publication.initialPage1187es
dc.publication.endPage1200es

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