dc.creator | Gaffield, Michael A. | es |
dc.creator | Tabares, Lucía | es |
dc.creator | Betz, William J. | es |
dc.date.accessioned | 2023-05-18T13:10:00Z | |
dc.date.available | 2023-05-18T13:10:00Z | |
dc.date.issued | 2009 | |
dc.identifier.citation | Gaffield, M.A., Tabares, L. y Betz, W.J. (2009). Preferred Sites of Exocytosis and Endocytosis Colocalize during High- But Not Lower-Frequency Stimulation in Mouse Motor Nerve Terminals. JOURNAL OF NEUROSCIENCE, 29 (48), 15308-15316. https://doi.org/10.1523/JNEUROSCI.4646-09.2009. | |
dc.identifier.issn | 0270-6474 | es |
dc.identifier.uri | https://hdl.handle.net/11441/146354 | |
dc.description.abstract | The spatial relationship of exocytosis and endocytosis in motor nerve terminals has been explored, with varied results, mostly in fixed
preparations and without direct information on the utilization of each exocytic site. We sought to determine these spatial properties in
real time using synaptopHluorin (spH) and FM4-64. Earlier we showed that nerve stimulation elicits the appearance of spH fluorescence
hot spots, which mark preferred sites of exocytosis. Here we show that nerve stimulation in the presence of the styryl dye FM4-64 evokes
hot spots of FM4-64 fluorescence. Their size, density, and rate of appearance are similar to the spH hot spots, but their rate of disappearance after stimulation was much slower (t1/2 9 min vs 10 s for spH hot spots), consistent with FM4-64 spots identifying bulk
endocytosis and subsequent slow intracellular dispersion of nascent vesicles. Simultaneous imaging of both fluorophores revealed a
strong colocalization of spH and FM4-64 spots, but only during high (100 Hz) stimulation. At 40 Hz stimulation, exocytic and endocytic
spots did not colocalize. Our results are consistent with the hypothesis that hot spots of endocytosis, possibly in the form of bulk uptake,
occur at or very near highly active exocytic sites during high-frequency stimulation. | es |
dc.format | application/pdf | es |
dc.format.extent | 9 p. | es |
dc.language.iso | eng | es |
dc.publisher | SOC NEUROSCIENCE | es |
dc.relation.ispartof | JOURNAL OF NEUROSCIENCE, 29 (48), 15308-15316. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Preferred Sites of Exocytosis and Endocytosis Colocalize during High- But Not Lower-Frequency Stimulation in Mouse Motor Nerve Terminals | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Fisiología Médica y Biofísica | es |
dc.relation.publisherversion | https://www.jneurosci.org/content/29/48/15308 | es |
dc.identifier.doi | 10.1523/JNEUROSCI.4646-09.2009 | es |
dc.journaltitle | JOURNAL OF NEUROSCIENCE | es |
dc.publication.volumen | 29 | es |
dc.publication.issue | 48 | es |
dc.publication.initialPage | 15308 | es |
dc.publication.endPage | 15316 | es |