Article
Particle Finder: a Simple Particle Detection Tool for Continuous-flow Systems
Author/s | Fernández Mateo, Raúl
Calero, Víctor García Sánchez, Pablo Ramos Reyes, Antonio Morgan, Hywel |
Department | Universidad de Sevilla. Departamento de Electrónica y Electromagnetismo |
Publication Date | 2023 |
Deposit Date | 2023-05-11 |
Published in |
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Abstract | We describe a user-friendly, open source software for single-particle detection/counting in a continuous-flow. The tool automatically processes video images of particles, including pre-conditioning, followed by size-based ... We describe a user-friendly, open source software for single-particle detection/counting in a continuous-flow. The tool automatically processes video images of particles, including pre-conditioning, followed by size-based discrimination for independent detection of fluorescent and non-fluorescent particles of different sizes. This is done by interactive tuning of a reduced set of parameters that can be checked with a robust, real-time quality control of the original video files. The software provides a concentration distribution of the particles in the transverse direction of the fluid flow. The software is a versatile tool for many microfluidic applications and does not require expertise in image analysis. |
Citation | Fernández Mateo, R., Calero, V., García Sánchez, P., Ramos Reyes, A. y Morgan, H. (2023). Particle Finder: a Simple Particle Detection Tool for Continuous-flow Systems. Microfluidics and Nanofluidics, 27 (3), 19. https://doi.org/10.1007/s10404-023-02626-7. |
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