dc.creator | Cermeño, María | es |
dc.creator | Bascón Suárez, María del Carmen | es |
dc.creator | Amigo-Benavent, Miryam | es |
dc.creator | Félix Ángel, Manuel | es |
dc.creator | FitzGerald, Richard J. | es |
dc.date.accessioned | 2023-01-03T11:11:56Z | |
dc.date.available | 2023-01-03T11:11:56Z | |
dc.date.issued | 2022-05 | |
dc.identifier.citation | Cermeño, M., Bascón Suárez, M.d.C., Amigo-Benavent, M., Félix Ángel, M. y FitzGerald, R.J. (2022). Identification of peptides from edible silkworm pupae (Bombyx mori) protein hydrolysates with antioxidant activity. Journal of Functional Foods, 92 (105052). https://doi.org/10.1016/j.jff.2022.105052. | |
dc.identifier.issn | 1756-4646 | es |
dc.identifier.uri | https://hdl.handle.net/11441/140889 | |
dc.description.abstract | Silkworm (Bombyx mori) pupae is a by-product from the silk industry which is rich in protein. Hydrolysates from
silkworm pupae generated using Alcalase®, Prolyve®, Flavourzyme® and Brewers Clarex® proteolytic prepa-
rations were characterised. The antioxidant activity of the hydrolysates was investigated using in vitro antioxi-
dant assays and an in situ assay for reactive oxygen species (ROS) reduction using hepatic HepG2. Overall,
Alcalase and Prolyve hydrolysates had highest scavenging activities, however, Flavourzyme and Brewers Clarex
hydrolysates had enhanced ferric reducing antioxidant power (FRAP) activity compared to the other samples.
Furthermore, the Flavourzyme hydrolysate significantly reduced ROS by 40% compared to untreated control
HepG2 cells. Peptides identified by LC-MS/MS were synthetised and then tested for their in vitro and in situ
antioxidant activity. Peptides SWFVTPF and NDVLFF showed highest antioxidant activity (ROS reduction, su-
peroxide dismutase (SOD) expression and glutathione (GSH) production activity) in HepG2 cells, and therefore
may have potential as natural antioxidants. | es |
dc.format | application/pdf | es |
dc.format.extent | 10 p. | es |
dc.language.iso | eng | es |
dc.publisher | Elsevier | es |
dc.relation.ispartof | Journal of Functional Foods, 92 (105052). | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Antioxidant | es |
dc.subject | Bioactive | es |
dc.subject | Bombyx mori | es |
dc.subject | Peptide | es |
dc.subject | Silkworm | es |
dc.title | Identification of peptides from edible silkworm pupae (Bombyx mori) protein hydrolysates with antioxidant activity | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Ingeniería Química | es |
dc.relation.projectID | DT20180088 | es |
dc.relation.projectID | VPPI-US Ref.-II.5 | es |
dc.relation.projectID | PY20_01046 | es |
dc.relation.publisherversion | https://www.sciencedirect.com/science/article/pii/S1756464622001220 | es |
dc.identifier.doi | 10.1016/j.jff.2022.105052 | es |
dc.contributor.group | Universidad de Sevilla. TEP229: Tecnología y Diseño de Productos Multicomponentes | es |
dc.journaltitle | Journal of Functional Foods | es |
dc.publication.volumen | 92 | es |
dc.publication.issue | 105052 | es |
dc.contributor.funder | The Disruptive Technologies Innovation Fund by Enterprise Ireland (DTIF) grant no. DT20180088 | es |
dc.contributor.funder | University of Seville for the VPPI-US grant Ref.-II.5 | es |
dc.contributor.funder | Consejería de Transformación económica, Industria, Conocimiento y Universidades de la Junta de Andalucía-Agencia Andaluza del Conocimiento and FEDER, UE by the grant Ref. PY20_01046 | es |