dc.creator | Ramírez-Ponce, M. Pilar | es |
dc.creator | Sola García, Alejandro | es |
dc.creator | Balseiro Gómez, Santiago | es |
dc.creator | Maldonado y Aibar, María Dolores | es |
dc.creator | Acosta López, Jorge | es |
dc.creator | Alés González de la Higuera, Eva María | es |
dc.creator | Flores Cordero, Juan Manuel | es |
dc.date.accessioned | 2022-09-07T15:18:09Z | |
dc.date.available | 2022-09-07T15:18:09Z | |
dc.date.issued | 2021 | |
dc.identifier.citation | Ramírez-Ponce, M.P., Sola García, A., Balseiro Gómez, S., Maldonado y Aibar, M.D., Acosta López, J., Alés González de la Higuera, E.M. y Flores Cordero, J.M. (2021). Mast Cell Changes the Phenotype of Microglia via Histamine and ATP. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 55 (1), 17-32. | |
dc.identifier.issn | 1015-8987 | es |
dc.identifier.uri | https://hdl.handle.net/11441/136861 | |
dc.description.abstract | Background/Aims: Microglia are the dynamic motile phagocytes of the brain considered
the first line of defense against threats or disturbances to the Central Nervous System
(CNS). Microglia help orchestrate the immunological response by interacting with others
immune cells. Mast cells (MCs) are effector cells of the innate immune system distributed
in all organs and vascularized tissues, brain included. Several molecular mechanisms for
potential interactions between MCs and microglia have been determined. However, the
effect of MCs on regulated exocytosis and phagocytic clearance in microglia has not been
explored. Methods: Cocktails of MCs mediators (MCM) obtained at 37°C and 53°C were used
to induce microglia activation. Changes in intracellular calcium [Ca2+]i and ATP release were
studied by calcium and quinacrine fluorescence imaging. Fluorescent latex beads were used
to assay phagocytosis in microglia after MCM treatment and compared to that measured
in the presence of histamine, ATP and lipopolysaccharide (LPS). Iba-1 expression and area
were quantified by immunofluorescence and histamine levels evaluated by ELISA techniques.
Results: Local application onto microglia of the MC mediator cocktail elicited Ca2+ transients
and exocytotic release associated with quinacrine dye de-staining. Ca2+ signals were mimicked
by histamine and blocked by the H1 receptor (H1R) antagonist, cetirizine. Hydrolysis of ATP
by apyrase also affected Ca2+ transients to a lesser extent. Iba-1 fluorescence, cell area and
phagocytosis were enhanced by histamine through H1R. However, ATP prevented iba-1
expression and microglial phagocytosis. MCM showed combined effects of histamine and
ATP, increasing the number of internalized microbeads per cell and area without raising iba1
expression. Conclusion: Our results highlight the relevance of MC-derived histamine and ATP
in the modulation of secretory and phagocytic activities that would explain the heterogeneity
of microglial responses in different pathological contexts. | es |
dc.description.sponsorship | Agencia Estatal de Investigación/Proyecto | es |
dc.description.sponsorship | Junta de Andalucía | es |
dc.format | application/pdf | es |
dc.format.extent | 16 p. | es |
dc.language.iso | eng | es |
dc.publisher | KARGER | es |
dc.relation.ispartof | CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 55 (1), 17-32. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Exocytosis | es |
dc.subject | Phagocytosis | es |
dc.subject | Histamine | es |
dc.subject | ATP | es |
dc.subject | Mast cells | es |
dc.subject | Microglia | es |
dc.title | Mast Cell Changes the Phenotype of Microglia via Histamine and ATP | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Fisiología Médica y Biofísica | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Bioquímica Médica y Biología Molecular e Inmunología | es |
dc.relation.projectID | BFU2017-85832R | es |
dc.relation.projectID | BIO-236 | es |
dc.relation.publisherversion | https://www.cellphysiolbiochem.com/Articles/000324/ | es |
dc.identifier.doi | 10.33594/000000324 | es |
dc.journaltitle | CELLULAR PHYSIOLOGY AND BIOCHEMISTRY | es |
dc.publication.volumen | 55 | es |
dc.publication.issue | 1 | es |
dc.publication.initialPage | 17 | es |
dc.publication.endPage | 32 | es |