dc.creator | Rodríguez Gallardo, Sofía | es |
dc.creator | Sabido Bozo, Susana | es |
dc.creator | Ikeda, Atsuko | es |
dc.creator | Araki, Misako | es |
dc.creator | Okazaki, Kouta | es |
dc.creator | Nakano, Miyako | es |
dc.creator | Aguilera Romero, María Auxiliadora | es |
dc.creator | Cortés Gómez, Alejandro | es |
dc.creator | López Martín, Sergio | es |
dc.creator | Waga, Miho | es |
dc.creator | Nakano, Akihiko | es |
dc.creator | Kurokawa, Kazuo | es |
dc.creator | Muñiz Guinea, Manuel | es |
dc.creator | Funato, Kouichi | es |
dc.date.accessioned | 2022-05-23T15:21:35Z | |
dc.date.available | 2022-05-23T15:21:35Z | |
dc.date.issued | 2022 | |
dc.identifier.citation | Rodríguez Gallardo, S., Sabido Bozo, S., Ikeda, A., Araki, M., Okazaki, K., Nakano, M.,...,Funato, K. (2022). Quality-controlled ceramide-based GPI-anchored protein sorting into selective ER exit sites. Cell Reports, 39 (5), 110768. | |
dc.identifier.issn | 2211-1247 | es |
dc.identifier.uri | https://hdl.handle.net/11441/133541 | |
dc.description.abstract | Glycosylphosphatidylinositol-anchored proteins (GPI-APs) exit the endoplasmic reticulum (ER) through a specialized export pathway in the yeast Saccharomyces cerevisiae. We have recently shown that a very-long acyl chain (C26) ceramide present in the ER membrane drives clustering and sorting of GPI-APs into selective ER exit sites (ERES). Now, we show that this lipid-based ER sorting also involves the C26 ceramide as a lipid moiety of GPI-APs, which is incorporated into the GPI anchor through a lipid-remodeling process after protein attachment in the ER. Moreover, we also show that a GPI-AP with a C26 ceramide moiety is monitored by the GPI-glycan remodelase Ted1, which, in turn, is required for receptor-mediated export of GPI-APs. Therefore, our study reveals a quality-control system that ensures lipid-based sorting of GPI-APs into selective ERESs for differential ER export, highlighting the physiological need for this specific export pathway. | es |
dc.description.sponsorship | Japan Society for the Promotion of Science JP19H02922, JP25221103, JP17H06420, JP18H0527 | es |
dc.description.sponsorship | Ministerio de Ciencia e Innovación PID2020-119505GB-I00, AEI/10.13039/501100011033 | es |
dc.description.sponsorship | Junta de Andalucía ERDF PY20_01240, US- 1380893 | es |
dc.description.sponsorship | Universidad de Sevilla VIPPIT-2020-I.5 | es |
dc.format | application/pdf | es |
dc.format.extent | 16 p. | es |
dc.language.iso | eng | es |
dc.publisher | Elsevier | es |
dc.relation.ispartof | Cell Reports, 39 (5), 110768. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Ceramide remodeling | es |
dc.subject | CP: Cell biology | es |
dc.subject | CP: Molecular biology | es |
dc.subject | Endoplasmic reticulum | es |
dc.subject | Glycan remodeling | es |
dc.subject | GPI-anchored protein | es |
dc.subject | Protein sorting | es |
dc.subject | Quality control | es |
dc.subject | Yeast Saccharomyces cerevisiae | es |
dc.title | Quality-controlled ceramide-based GPI-anchored protein sorting into selective ER exit sites | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Biología Celular | es |
dc.relation.projectID | JP19H02922 | es |
dc.relation.projectID | JP25221103 | es |
dc.relation.projectID | JP17H06420 | es |
dc.relation.projectID | JP18H0527 | es |
dc.relation.projectID | PID2020-119505GB-I00 | es |
dc.relation.projectID | AEI/10.13039/501100011033 | es |
dc.relation.projectID | ERDF PY20_01240 | es |
dc.relation.projectID | US- 1380893 | es |
dc.relation.projectID | VIPPIT-2020-I.5 | es |
dc.relation.publisherversion | https://doi.org/10.1016/j.celrep.2022.110768 | es |
dc.identifier.doi | 10.1016/j.celrep.2022.110768 | es |
dc.journaltitle | Cell Reports | es |
dc.publication.volumen | 39 | es |
dc.publication.issue | 5 | es |
dc.publication.initialPage | 110768 | es |
dc.contributor.funder | Society for the Promotion of Science. Japan | es |
dc.contributor.funder | Agencia Estatal de Investigación. España | es |
dc.contributor.funder | Junta de Andalucía | es |
dc.contributor.funder | Universidad de Sevilla | es |
dc.description.awardwinning | Premio Anual Publicación Científica Destacada de la US. Facultad de Biología | |