Artículo
Quality-controlled ceramide-based GPI-anchored protein sorting into selective ER exit sites
Autor/es | Rodríguez Gallardo, Sofía
Sabido Bozo, Susana Ikeda, Atsuko Araki, Misako Okazaki, Kouta Nakano, Miyako Aguilera Romero, María Auxiliadora Cortés Gómez, Alejandro López Martín, Sergio Waga, Miho Nakano, Akihiko Kurokawa, Kazuo Muñiz Guinea, Manuel Funato, Kouichi |
Departamento | Universidad de Sevilla. Departamento de Biología Celular |
Fecha de publicación | 2022 |
Fecha de depósito | 2022-05-23 |
Publicado en |
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Premios | Premio Anual Publicación Científica Destacada de la US. Facultad de Biología |
Resumen | Glycosylphosphatidylinositol-anchored proteins (GPI-APs) exit the endoplasmic reticulum (ER) through a specialized export pathway in the yeast Saccharomyces cerevisiae. We have recently shown that a very-long acyl chain ... Glycosylphosphatidylinositol-anchored proteins (GPI-APs) exit the endoplasmic reticulum (ER) through a specialized export pathway in the yeast Saccharomyces cerevisiae. We have recently shown that a very-long acyl chain (C26) ceramide present in the ER membrane drives clustering and sorting of GPI-APs into selective ER exit sites (ERES). Now, we show that this lipid-based ER sorting also involves the C26 ceramide as a lipid moiety of GPI-APs, which is incorporated into the GPI anchor through a lipid-remodeling process after protein attachment in the ER. Moreover, we also show that a GPI-AP with a C26 ceramide moiety is monitored by the GPI-glycan remodelase Ted1, which, in turn, is required for receptor-mediated export of GPI-APs. Therefore, our study reveals a quality-control system that ensures lipid-based sorting of GPI-APs into selective ERESs for differential ER export, highlighting the physiological need for this specific export pathway. |
Agencias financiadoras | Society for the Promotion of Science. Japan Agencia Estatal de Investigación. España Junta de Andalucía Universidad de Sevilla |
Identificador del proyecto | JP19H02922
JP25221103 JP17H06420 JP18H0527 PID2020-119505GB-I00 AEI/10.13039/501100011033 ERDF PY20_01240 US- 1380893 VIPPIT-2020-I.5 |
Cita | Rodríguez Gallardo, S., Sabido Bozo, S., Ikeda, A., Araki, M., Okazaki, K., Nakano, M.,...,Funato, K. (2022). Quality-controlled ceramide-based GPI-anchored protein sorting into selective ER exit sites. Cell Reports, 39 (5), 110768. |
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