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Quality-controlled ceramide-based GPI-anchored protein sorting into selective ER exit sites
Author/s | Rodríguez Gallardo, Sofía
![]() ![]() ![]() ![]() ![]() ![]() Sabido Bozo, Susana Ikeda, Atsuko Araki, Misako Okazaki, Kouta Nakano, Miyako Aguilera Romero, María Auxiliadora ![]() ![]() ![]() ![]() ![]() ![]() Cortés Gómez, Alejandro López Martín, Sergio ![]() ![]() ![]() ![]() ![]() ![]() Waga, Miho Nakano, Akihiko Kurokawa, Kazuo Muñiz Guinea, Manuel ![]() ![]() ![]() ![]() ![]() ![]() ![]() Funato, Kouichi |
Department | Universidad de Sevilla. Departamento de Biología Celular |
Publication Date | 2022 |
Deposit Date | 2022-05-23 |
Published in |
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Awards | Premio Anual Publicación Científica Destacada de la US. Facultad de Biología |
Abstract | Glycosylphosphatidylinositol-anchored proteins (GPI-APs) exit the endoplasmic reticulum (ER) through a specialized export pathway in the yeast Saccharomyces cerevisiae. We have recently shown that a very-long acyl chain ... Glycosylphosphatidylinositol-anchored proteins (GPI-APs) exit the endoplasmic reticulum (ER) through a specialized export pathway in the yeast Saccharomyces cerevisiae. We have recently shown that a very-long acyl chain (C26) ceramide present in the ER membrane drives clustering and sorting of GPI-APs into selective ER exit sites (ERES). Now, we show that this lipid-based ER sorting also involves the C26 ceramide as a lipid moiety of GPI-APs, which is incorporated into the GPI anchor through a lipid-remodeling process after protein attachment in the ER. Moreover, we also show that a GPI-AP with a C26 ceramide moiety is monitored by the GPI-glycan remodelase Ted1, which, in turn, is required for receptor-mediated export of GPI-APs. Therefore, our study reveals a quality-control system that ensures lipid-based sorting of GPI-APs into selective ERESs for differential ER export, highlighting the physiological need for this specific export pathway. |
Funding agencies | Society for the Promotion of Science. Japan Agencia Estatal de Investigación. España Junta de Andalucía Universidad de Sevilla |
Project ID. | JP19H02922
![]() JP25221103 ![]() JP17H06420 ![]() JP18H0527 ![]() PID2020-119505GB-I00 ![]() AEI/10.13039/501100011033 ![]() ERDF PY20_01240 ![]() US- 1380893 ![]() VIPPIT-2020-I.5 ![]() |
Citation | Rodríguez Gallardo, S., Sabido Bozo, S., Ikeda, A., Araki, M., Okazaki, K., Nakano, M.,...,Funato, K. (2022). Quality-controlled ceramide-based GPI-anchored protein sorting into selective ER exit sites. Cell Reports, 39 (5), 110768. |
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