dc.creator | Gallardo Fernández, Marta | es |
dc.creator | Valls Fonayet, J. | es |
dc.creator | Valero, E. | es |
dc.creator | Hornedo Ortega, Ruth | es |
dc.creator | Richard, T. | es |
dc.creator | Troncoso González, Ana María | es |
dc.creator | García Parrilla, María del Carmen | es |
dc.date.accessioned | 2022-03-24T08:38:03Z | |
dc.date.available | 2022-03-24T08:38:03Z | |
dc.date.issued | 2022 | |
dc.identifier.citation | Gallardo Fernández, M., Valls Fonayet, J., Valero, E., Hornedo Ortega, R., Richard, T., Troncoso González, A.M. y García Parrilla, M.d.C. (2022). Isotopic labelling-based analysis elucidates biosynthesis pathways in Saccharomyces cerevisiae for Melatonin, Serotonin and Hydroxytyrosol formation. Food Chemistry, 374, 131742. | |
dc.identifier.issn | 0308-8146 | es |
dc.identifier.uri | https://hdl.handle.net/11441/131223 | |
dc.description.abstract | Yeasts can synthetise bioactive compounds such as Melatonin (MEL), Serotonin (SER) and Hydroxytyrosol (HT). Deciphering the mechanisms involved in their formation can lead to exploit this fact to increase the bioactive potential of fermented beverages. Quantitative analysis using labelled compounds, 15-N2 l-tryptophan and 13-C tyrosine, allowed tracking the formation of the above-mentioned bioactive compounds during the alcoholic fermentation of synthetic must by two different Saccharomyces cerevisiae strains. Labelled and unlabelled MEL, SER and HT were undoubtedly identified and quantified by High Resolution Mass Spectrometry (HRMS). Our results prove that there are at least two pathways involved in MEL biosynthesis by yeast. One starts with tryptophan as precursor being known for the vertebrates’ pathway. Additionally, MEL is produced from SER which in turn is consistent with the plants’ biosynthesis pathway. Concerning HT, it can be formed both from labelled tyrosine and from intermediates of the Erlich pathway. | es |
dc.description.sponsorship | Ministerio de Economía y Competitividad AGL2016-77505-C3-2-R | es |
dc.description.sponsorship | Ministerio de Ciencia, Innovación y Universidades PID2019-108722RB-C32 | es |
dc.description.sponsorship | Junta de Andalucía, Consejería de Economía y Conocimiento P18-RT-3098 | es |
dc.format | application/pdf | es |
dc.format.extent | 10 p. | es |
dc.language.iso | eng | es |
dc.publisher | Elsevier | es |
dc.relation.ispartof | Food Chemistry, 374, 131742. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Yeast | es |
dc.subject | Nitrogen | es |
dc.subject | Indolic compounds | es |
dc.subject | Polyphenol | es |
dc.subject | Alcoholic fermentation | es |
dc.subject | HRMS | es |
dc.title | Isotopic labelling-based analysis elucidates biosynthesis pathways in Saccharomyces cerevisiae for Melatonin, Serotonin and Hydroxytyrosol formation | es |
dc.type | info:eu-repo/semantics/article | es |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Nutrición y Bromatología, Toxicología y Medicina Legal | es |
dc.relation.projectID | AGL2016-77505-C3-2-R | es |
dc.relation.projectID | PID2019-108722RB-C32 | es |
dc.relation.projectID | P18-RT-3098 | es |
dc.relation.publisherversion | http://dx.doi.org/10.1016/j.foodchem.2021.131742 | es |
dc.identifier.doi | 10.1016/j.foodchem.2021.131742 | es |
dc.journaltitle | Food Chemistry | es |
dc.publication.volumen | 374 | es |
dc.publication.initialPage | 131742 | es |
dc.contributor.funder | Ministerio de Economía y Competitividad (MINECO). España | es |
dc.contributor.funder | Ministerio de Ciencia, Innovación y Universidades (MICINN). España | es |
dc.contributor.funder | Junta de Andalucía | es |
dc.description.awardwinning | Premio Mensual Publicación Científica Destacada de la US. Facultad de Farmacia | |