dc.creator | Rodríguez Olivenza, David | es |
dc.creator | Casadesús Pursals, Josep | es |
dc.creator | Ansaldi, Mireille | es |
dc.date.accessioned | 2022-03-21T12:23:11Z | |
dc.date.available | 2022-03-21T12:23:11Z | |
dc.date.issued | 2020 | |
dc.identifier.citation | Rodríguez Olivenza, D., Casadesús Pursals, J. y Ansaldi, M. (2020). Epigenetic biosensors for bacteriophage detection and phage receptor discrimination. Environmental Microbiology, 22 (8), 3126-3142. | |
dc.identifier.issn | 1462-2912 | es |
dc.identifier.issn | 1462-2920 | es |
dc.identifier.uri | https://hdl.handle.net/11441/131092 | |
dc.description.abstract | Environmental monitoring of bacteria using phage-based biosensors has been widely developed for many different species. However, there are only a few available methods to detect specific bacteriophages in raw environmental samples. In this work, we developed a simple and efficient assay to rapidly monitor the phage content of a given sample. The assay is based on the bistable expression of the Salmonella enterica opvAB operon. Under regular growth conditions, opvAB is only expressed by a small fraction of the bacterial subpopulation. In the OpvABON subpopulation, synthesis of the OpvA and OpvB products shortens the O-antigen and confers resistance to phages that use LPS as a receptor. As a consequence, the OpvABON subpopulation is selected in the presence of such phages. Using an opvAB::gfp fusion, we could monitor LPS-binding phages in various media, including raw water samples. To enlarge our phage-biosensor panoply, we also developed biosensors able to detect LPS, as well as protein-binding coliphages. Moreover, the combination of these tools allowed to identify the bacterial receptor triggering phage infection. The epigenetic opvAB::gfp biosensor thus comes in different flavours to detect a wide range of bacteriophages and identify the type of receptor they recognize. | es |
dc.description.sponsorship | Ministerio de Ciencia, Innovación y Universidades de España. BIO2016-75235-P | es |
dc.description.sponsorship | Agence Nationale de la Recherche de Francia. ANR-16-CE12-0029-01 | es |
dc.format | application/pdf | es |
dc.format.extent | 17 p. | es |
dc.language.iso | eng | es |
dc.publisher | Society for Applied Microbiology | es |
dc.relation.ispartof | Environmental Microbiology, 22 (8), 3126-3142. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Epigenetic biosensors for bacteriophage detection and phage receptor discrimination | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Genética | es |
dc.relation.projectID | BIO2016-75235-P | es |
dc.relation.projectID | ANR-16-CE12-0029-01 | es |
dc.relation.publisherversion | https://doi.org/10.1111/1462-2920.15050 | es |
dc.identifier.doi | 10.1111/1462-2920.15050 | es |
dc.journaltitle | Environmental Microbiology | es |
dc.publication.volumen | 22 | es |
dc.publication.issue | 8 | es |
dc.publication.initialPage | 3126 | es |
dc.publication.endPage | 3142 | es |
dc.contributor.funder | Ministerio de Ciencia, Innovación y Universidades (MICINN). España | es |
dc.contributor.funder | Agence Nationale de la Recherche. France | es |