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dc.creatorRodríguez Olivenza, Davides
dc.creatorCasadesús Pursals, Josepes
dc.creatorAnsaldi, Mireillees
dc.date.accessioned2022-03-21T12:23:11Z
dc.date.available2022-03-21T12:23:11Z
dc.date.issued2020
dc.identifier.citationRodríguez Olivenza, D., Casadesús Pursals, J. y Ansaldi, M. (2020). Epigenetic biosensors for bacteriophage detection and phage receptor discrimination. Environmental Microbiology, 22 (8), 3126-3142.
dc.identifier.issn1462-2912es
dc.identifier.issn1462-2920es
dc.identifier.urihttps://hdl.handle.net/11441/131092
dc.description.abstractEnvironmental monitoring of bacteria using phage-based biosensors has been widely developed for many different species. However, there are only a few available methods to detect specific bacteriophages in raw environmental samples. In this work, we developed a simple and efficient assay to rapidly monitor the phage content of a given sample. The assay is based on the bistable expression of the Salmonella enterica opvAB operon. Under regular growth conditions, opvAB is only expressed by a small fraction of the bacterial subpopulation. In the OpvABON subpopulation, synthesis of the OpvA and OpvB products shortens the O-antigen and confers resistance to phages that use LPS as a receptor. As a consequence, the OpvABON subpopulation is selected in the presence of such phages. Using an opvAB::gfp fusion, we could monitor LPS-binding phages in various media, including raw water samples. To enlarge our phage-biosensor panoply, we also developed biosensors able to detect LPS, as well as protein-binding coliphages. Moreover, the combination of these tools allowed to identify the bacterial receptor triggering phage infection. The epigenetic opvAB::gfp biosensor thus comes in different flavours to detect a wide range of bacteriophages and identify the type of receptor they recognize.es
dc.description.sponsorshipMinisterio de Ciencia, Innovación y Universidades de España. BIO2016-75235-Pes
dc.description.sponsorshipAgence Nationale de la Recherche de Francia. ANR-16-CE12-0029-01es
dc.formatapplication/pdfes
dc.format.extent17 p.es
dc.language.isoenges
dc.publisherSociety for Applied Microbiologyes
dc.relation.ispartofEnvironmental Microbiology, 22 (8), 3126-3142.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleEpigenetic biosensors for bacteriophage detection and phage receptor discriminationes
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Genéticaes
dc.relation.projectIDBIO2016-75235-Pes
dc.relation.projectIDANR-16-CE12-0029-01es
dc.relation.publisherversionhttps://doi.org/10.1111/1462-2920.15050es
dc.identifier.doi10.1111/1462-2920.15050es
dc.journaltitleEnvironmental Microbiologyes
dc.publication.volumen22es
dc.publication.issue8es
dc.publication.initialPage3126es
dc.publication.endPage3142es
dc.contributor.funderMinisterio de Ciencia, Innovación y Universidades (MICINN). Españaes
dc.contributor.funderAgence Nationale de la Recherche. Francees

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