Epigenetic biosensors for bacteriophage detection and phage receptor discrimination
|Author/s||Rodríguez Olivenza, David
Casadesús Pursals, Josep
|Department||Universidad de Sevilla. Departamento de Genética|
|Abstract||Environmental monitoring of bacteria using phage-based biosensors has been widely developed for many different species. However, there are only a few available methods to detect specific bacteriophages in raw environmental ...
Environmental monitoring of bacteria using phage-based biosensors has been widely developed for many different species. However, there are only a few available methods to detect specific bacteriophages in raw environmental samples. In this work, we developed a simple and efficient assay to rapidly monitor the phage content of a given sample. The assay is based on the bistable expression of the Salmonella enterica opvAB operon. Under regular growth conditions, opvAB is only expressed by a small fraction of the bacterial subpopulation. In the OpvABON subpopulation, synthesis of the OpvA and OpvB products shortens the O-antigen and confers resistance to phages that use LPS as a receptor. As a consequence, the OpvABON subpopulation is selected in the presence of such phages. Using an opvAB::gfp fusion, we could monitor LPS-binding phages in various media, including raw water samples. To enlarge our phage-biosensor panoply, we also developed biosensors able to detect LPS, as well as protein-binding coliphages. Moreover, the combination of these tools allowed to identify the bacterial receptor triggering phage infection. The epigenetic opvAB::gfp biosensor thus comes in different flavours to detect a wide range of bacteriophages and identify the type of receptor they recognize.
|Funding agencies||Ministerio de Ciencia, Innovación y Universidades (MICINN). España
Agence Nationale de la Recherche. France
|Citation||Rodríguez Olivenza, D., Casadesús Pursals, J. y Ansaldi, M. (2020). Epigenetic biosensors for bacteriophage detection and phage receptor discrimination. Environmental Microbiology, 22 (8), 3126-3142.|