dc.creator | Montes Fernández, María de los Ángeles | es |
dc.creator | Pérez Villegas, Eva María | es |
dc.creator | García-Gonzalo, Francesc R. | es |
dc.creator | Pedrazza, Leonardo | es |
dc.creator | Rosa, José Luis | es |
dc.creator | Álvarez de Toledo Naranjo, Guillermo | es |
dc.creator | Armengol, José Ángel | es |
dc.date.accessioned | 2021-08-30T10:38:12Z | |
dc.date.available | 2021-08-30T10:38:12Z | |
dc.date.issued | 2020-07-21 | |
dc.identifier.citation | Montes Fernández, M.d.l.Á., Pérez Villegas, E.M., García-Gonzalo, F.R., Pedrazza, L., Rosa, J.L., Álvarez de Toledo Naranjo, G. y Armengol, J.A. (2020). The HERC1 ubiquitin ligase regulates presynaptic membrane dynamics of central synapses. Scientific Reports, 10 (1), art.n.12057. | |
dc.identifier.issn | 2045-2322 | es |
dc.identifier.uri | https://hdl.handle.net/11441/125214 | |
dc.description.abstract | HERC1 is a ubiquitin ligase protein, which, when mutated, induces several malformations and
intellectual disability in humans. The animal model of HERC1 mutation is the mouse tambaleante
characterized by: (1) overproduction of the protein; (2) cerebellar Purkinje cells death by autophagy;
(3) dysregulation of autophagy in spinal cord motor neurons, and CA3 and neocortical pyramidal
neurons; (4) impairment of associative learning, linked to altered spinogenesis and absence of LTP
in the lateral amygdala; and, (5) motor impairment due to delayed action potential transmission,
decrease synaptic transmission efciency and altered myelination in the peripheral nervous system.
To investigate the putative role of HERC1 in the presynaptic dynamics we have performed a series
of experiments in cultured tambaleante hippocampal neurons by using transmission electron
microscopy, FM1-43 destaining and immunocytochemistry. Our results show: (1) a decrease in the
number of synaptic vesicles; (2) reduced active zones; (3) less clathrin immunoreactivity and less
presynaptic endings over the hippocampal main dendritic trees; which contrast with (4) a greater
number of endosomes and autophagosomes in the presynaptic endings of the tambaleante neurons
relative to control ones. Altogether these results show an important role of HERC1 in the regulation of
presynaptic membrane dynamics. | es |
dc.description.sponsorship | Junta de Andalucía BIO-209 | es |
dc.description.sponsorship | MINECO-DGICYT BFU2015-64536-R | es |
dc.description.sponsorship | Junta de Andalucía BIO-122 | es |
dc.description.sponsorship | MINECO-DGICYT BFU2015-64536-R | es |
dc.description.sponsorship | MINECO-AEI/FEDER, UE, BFU2016-80295-R | es |
dc.format | application/pdf | es |
dc.format.extent | 19 p. | es |
dc.language.iso | eng | es |
dc.publisher | Nature Research | es |
dc.relation.ispartof | Scientific Reports, 10 (1), art.n.12057. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Ubiquitin | es |
dc.subject | HERC1 | es |
dc.subject | Synaptic vesicles | es |
dc.subject | Central synapses | es |
dc.title | The HERC1 ubiquitin ligase regulates presynaptic membrane dynamics of central synapses | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Fisiología Médica y Biofísica | es |
dc.relation.projectID | BIO-209 | es |
dc.relation.projectID | BFU2015-64536-R | es |
dc.relation.projectID | BIO-122 | es |
dc.relation.projectID | BFU2015-64536-R | es |
dc.relation.projectID | BFU2016-80295-R | es |
dc.relation.publisherversion | https://www.nature.com/articles/s41598-020-68970-8 | es |
dc.identifier.doi | 10.1038/s41598-020-68970-8 | es |
dc.journaltitle | Scientific Reports | es |
dc.publication.volumen | 10 | es |
dc.publication.issue | 1 | es |
dc.publication.initialPage | art.n.12057 | es |