dc.creator | Flores Cordero, Juan Antonio | es |
dc.creator | Balseiro Gómez, Santiago | es |
dc.creator | Cabeza, Jose M. | es |
dc.creator | Acosta López, Jorge | es |
dc.creator | Ramírez-Ponce, M. Pilar | es |
dc.creator | Alés González de la Higuera, Eva María | es |
dc.date.accessioned | 2021-08-23T11:14:43Z | |
dc.date.available | 2021-08-23T11:14:43Z | |
dc.date.issued | 2014-06-24 | |
dc.identifier.citation | Flores Cordero, J.A., Balseiro-Gomez, S., Cabeza, J.M., Acosta, J., Ramirez-Ponce, P. y Alés González de la Higuera, E.M. (2014). A New Role for Myosin II in Vesicle Fission. PLoS ONE, 9 (6), art.n.e100757. | |
dc.identifier.issn | 1932-6203 (electrónico) | es |
dc.identifier.uri | https://hdl.handle.net/11441/125144 | |
dc.description.abstract | An endocytic vesicle is formed from a flat plasma membrane patch by a sequential process of invagination, bud formation
and fission. The scission step requires the formation of a tubular membrane neck (the fission pore) that connects the
endocytic vesicle with the plasma membrane. Progress in vesicle fission can be measured by the formation and closure of
the fission pore. Live-cell imaging and sensitive biophysical measurements have provided various glimpses into the
structure and behaviour of the fission pore. In the present study, the role of non-muscle myosin II (NM-2) in vesicle fission
was tested by analyzing the kinetics of the fission pore with perforated-patch clamp capacitance measurements to detect
single vesicle endocytosis with millisecond time resolution in peritoneal mast cells. Blebbistatin, a specific inhibitor of NM-2,
dramatically increased the duration of the fission pore and also prevented closure during large endocytic events. Using the
fluorescent markers FM1-43 and pHrodo Green dextran, we found that NM-2 inhibition greatly arrested vesicle fission in a
late phase of the scission event when the pore reached a final diameter of , 5 nm. Our results indicate that loss of the
ATPase activity of myosin II drastically reduces the efficiency of membrane scission by making vesicle c | es |
dc.description.sponsorship | Ministerio de Ciencia e Innovación ISCIII and Fondos FEDER Grant PI11/00257 | es |
dc.format | application/pdf | es |
dc.format.extent | 14 p. | es |
dc.language.iso | eng | es |
dc.publisher | Public Library of Science | es |
dc.relation.ispartof | PLoS ONE, 9 (6), art.n.e100757. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Myosin II | es |
dc.subject | Vesicle | es |
dc.subject | Blebbistatin | es |
dc.title | A New Role for Myosin II in Vesicle Fission | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Fisiología Médica y Biofísica | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Bioquímica Médica y Biología Molecular e Inmunología | es |
dc.relation.projectID | Grant PI11/00257 | es |
dc.relation.publisherversion | https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0100757 | es |
dc.identifier.doi | 10.1371/journal.pone.0100757 | es |
dc.journaltitle | PLoS ONE | es |
dc.publication.volumen | 9 | es |
dc.publication.issue | 6 | es |
dc.publication.initialPage | art.n.e100757 | es |