dc.creator | Fernández-Espejo, Emilio | es |
dc.creator | Rodríguez de Fonseca, Fernando | es |
dc.creator | Suárez, Juan | es |
dc.creator | Tolosa, Eduardo | es |
dc.creator | Vilas, Dolores | es |
dc.creator | Aldecoa, Iban | es |
dc.date.accessioned | 2021-07-13T11:25:19Z | |
dc.date.available | 2021-07-13T11:25:19Z | |
dc.date.issued | 2021-05-01 | |
dc.identifier.citation | Fernández-Espejo, E., Rodríguez de Fonseca, F., Suárez, J., Tolosa, E., Vilas, D. y Aldecoa, I. (2021). Native α-synuclein, 3-nitrotyrosine proteins, and patterns of nitro-α-synuclein-immunoreactive inclusions in saliva and submandibulary gland in parkinson’s disease. Antioxidants, 10 (5) | |
dc.identifier.issn | 2076-3921(electrónico) | es |
dc.identifier.uri | https://hdl.handle.net/11441/116057 | |
dc.description.abstract | Background. Salivary α-synuclein (aSyn) and its nitrated form, or 3-nitrotyrosine-αsynuclein (3-NT-αSyn), hold promise as biomarkers for idiopathic Parkinson’s disease (IPD). Nitrative
stress that is characterized by an excess of 3-nitrotyrosine proteins (3-NT-proteins) has been proposed
as a pathogenic mechanism in IPD. The objective is to study the pathological role of native αSyn,
3-NT-αSyn, and 3-NT-proteins in the saliva and submandibulary glands of patients with IPD. Methods.
The salivary and serum αSyn and 3-NT-proteins concentration is evaluated with ELISA in patients
and controls. Correlations of αSyn and 3-NT-proteins content with clinical features of the disease are
examined. Immunohistochemical 3-NT-αSyn expression in submandibulary gland sections is analyzed. Results. (a) Salivary concentration and saliva/serum ratios of native αSyn and 3-NT-proteins
are similar in patients and controls; (b) salivary αSyn and 3-NT-proteins do not correlate with any
clinical feature; and (c) three patterns of 3-NT-αSyn-positive inclusions are observed on histological
sections: rounded “Lewy-type” aggregates of 10–25 µm in diameter, coarse deposits with varied
morphology, and spheroid inclusions or bodies of 3–5 µm in diameter. “Lewy-type” and coarse
inclusions are observed in the interlobular connective tissue of the gland, and small-sized bodies are
located within the cytoplasm of duct cells. “Lewy-type” inclusions are only observed in patients,
and the remaining patterns of inclusions are observed in both the patients and controls. Conclusions.
The patients’ saliva presents a similar concentration of native αSyn and 3-nitrotyrosine-proteins than
that of the controls, and no correlations with clinical features are found. These findings preclude the
utility of native αSyn in the saliva as a biomarker, and they indicate the absence of nitrative stress in
the saliva and serum of patients. As regards nitrated αSyn, “Lewy-type” inclusions expressing 3-NTαSyn are observed in the patients, not the controls—a novel finding that suggests that a biopsy of the
submandibulary gland, if proven safe, could be a useful technique for diagnosing IPD. Finally, to our knowledge, this is also the first description of 3-NT-αSyn-immunoreactive intracytoplasmic bodies in
cells that are located outside the nervous system. These intracytoplasmic bodies are present in duct
cells of submandibulary gland sections from all subjects regardless of their pathology, and they can
represent an aging or involutional change. Further immunostaining studies with different antibodies
and larger samples are needed to validate the data. | es |
dc.description.sponsorship | Junta de Andalucía, Consejería de Economía, Conocimiento, Empresas y Universidad, España BIO127, PAIDI | es |
dc.description.sponsorship | Sociedad Andaluza de Neurología SUBAIA2015 / 006 | es |
dc.description.sponsorship | Fondos Europeos de Desarrollo Regional-Unión Europea y del Proyecto EULAC-HEALTH H2020 FATZHEIMER , EULACH16 / T010131 | es |
dc.description.sponsorship | Ministerio de Ciencia e Innovación, y FEDER- UE, RD16 / 0017/0001 e ISCIII, FEDER-UE PI19 / 01577 | es |
dc.description.sponsorship | Ministerio de Sanidad, Delegación de Gobierno para el Plan Nacional sobre Drogas, PND 2019/040) J.S. CPII17 / 00024 | es |
dc.format | application/pdf | es |
dc.format.extent | 14 p. | es |
dc.language.iso | eng | es |
dc.publisher | MDPI | es |
dc.relation.ispartof | Antioxidants, 10 (5) | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Parkinson | es |
dc.subject | A-synuclein | es |
dc.subject | Saliva | es |
dc.subject | Nitration | es |
dc.subject | Nitrative stress | es |
dc.subject | Lewy-type | es |
dc.title | Native α-synuclein, 3-nitrotyrosine proteins, and patterns of nitro-α-synuclein-immunoreactive inclusions in saliva and submandibulary gland in parkinson’s disease | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Fisiología Médica y Biofísica | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Medicina | es |
dc.relation.projectID | BIO127, PAIDI | es |
dc.relation.projectID | SUBAIA2015 / 006 | es |
dc.relation.projectID | RD16 / 0017/0001 | es |
dc.relation.projectID | PI19 / 01577 | es |
dc.relation.projectID | PND 2019/040 | es |
dc.relation.projectID | CPII17 / 00024 | es |
dc.relation.publisherversion | https://www.mdpi.com/journal/antioxidants | es |
dc.identifier.doi | 10.3390/antiox10050715 | es |
dc.journaltitle | Antioxidants | es |
dc.publication.volumen | 10 | es |
dc.publication.issue | 5 | es |