Cationic Single-Chained Surfactants with a Functional Group at the End of the Hydrophobic Tail DNA Compacting Efficiency
|Author||Lebrón Romero, José Antonio
López-Cornejo, María del Pilar
García Dionisio, Elena
Huertas Sánchez, Pablo
García Calderón, Margarita
Moyá Morán, María Luisa
Ostos Marcos, Francisco José
López López, Manuel
|Department||Universidad de Sevilla. Departamento de Química Física
Universidad de Sevilla. Departamento de Genética
Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular
|Published in||Pharmaceutics, 13 (4), 589.|
|Abstract||The interaction between calf-thymus DNA, ctDNA, and various single-chained surfactants with different functional groups at the end of hydrophobic tail was studied with the goal of investigating the influence of the functional ...
The interaction between calf-thymus DNA, ctDNA, and various single-chained surfactants with different functional groups at the end of hydrophobic tail was studied with the goal of investigating the influence of the functional group nature on surfactant DNA compacting efficiency. The surfactants investigated were dodecyltriethylammonium bromide (DTEABr), triethyl(1-phenoxydodecyl)ammonium bromide (12PhBr), triethyl(2-naphthoxydodecyl)ammonium bromide (12NBr) and 11-(isonicotinoyloxy)-N,N,N-triethyl-1-undecanaminium bromide (11PyBr). Results made evident that the surfactants’ tendencies to self-aggregation is the key factor determining their efficiency to compact the nucleic acid. Subsequently, DOPE/12NBr/pEGFP-C1 lipoplexes, with different cationic surfactant molar fractions (α) and mass ratios (L/D), were prepared and characterized. DOPE is a zwitterionic phospholipid 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine, and the plasmid pEGFP-C1 carries a GFP coding sequence with the necessary regulatory elements for constitutive expression of the gene in human cells. 12NBr was chosen because it was the most efficient DNA compacting agent among the surfactants investigated. Finally, the cytotoxicity and transfection efficiency (TE) of DOPE/12NBr/pDNA lipoplexes, with different compositions, were investigated.
|Cite||Lebrón Romero, J.A., López Cornejo, P., García Dionisio, E., Huertas Sánchez, P., García Calderón, M., Moyá Morán, M.L.,...,López López, M. (2021). Cationic Single-Chained Surfactants with a Functional Group at the End of the Hydrophobic Tail DNA Compacting Efficiency. Pharmaceutics, 13 (4), 589.|