dc.creator | Pérez-Puyana, Víctor Manuel | es |
dc.creator | Wieringa, Paul | es |
dc.creator | Yuste, Yaiza | es |
dc.creator | Portilla de Juan, Fernando de la | es |
dc.creator | Guerrero Conejo, Antonio Francisco | es |
dc.creator | Romero García, Alberto | es |
dc.creator | Moroni, Lorenzo | es |
dc.date.accessioned | 2021-06-02T07:14:19Z | |
dc.date.available | 2021-06-02T07:14:19Z | |
dc.date.issued | 2021-03 | |
dc.identifier.citation | Pérez-Puyana, V.M., Wieringa, P., Yuste, Y., Portilla de Juan, F.d.l., Guerrero Conejo, A.F., Romero García, A. y Moroni, L. (2021). Fabrication of hybrid scaffolds obtained from combinations of PCL with gelatin or collagen via electrospinning for skeletal muscle tissue engineering. Journal of Biomedical Materials Research part A, March, 1-13. | |
dc.identifier.issn | 1549-3296 | es |
dc.identifier.issn | 1552-4965 | es |
dc.identifier.uri | https://hdl.handle.net/11441/111277 | |
dc.description.abstract | The creation of skeletal muscle tissue in vitro is a major topic of interest today in the
field of biomedical research, due to the lack of treatments for muscle loss due to traumatic
accidents or disease. For this reason, the intrinsic properties of nanofibrillar structures
to promote cell adhesion, proliferation, and cell alignment presents an attractive
tool for regenerative medicine to recreate organized tissues such as muscle.
Electrospinning is one of the processing techniques often used for the fabrication of
these nanofibrous structures and the combination of synthetic and natural polymers is
often required to achieve optimal mechanical and physiochemical properties. Here,
polycaprolactone (PCL) is selected as a synthetic polymer used for the fabrication of
scaffolds, and the effect of protein addition on the final scaffolds' properties is studied.
Collagen and gelatin were the proteins selected and two different concentrations were
analyzed (2 and 4 wt/vol%). Different PCL/protein systems were prepared, and a structural,
mechanical and functional characterization was performed. The influence of fiber
alignment on the properties of the final scaffolds was assessed through morphological,
mechanical and biological evaluations. A bioreactor was used to promote cell proliferation
and differentiation within the scaffolds. The results revealed that protein addition
produced a decrease in the fiber size of the membranes, an increase in their hydrophilicity,
and a softening of their mechanical properties. The biological study showed the
ability of the selected systems to harbor cells, allow their growth and, potentially,
develop musculoskeletal tissues. | es |
dc.description.sponsorship | Ministerio de Economía y Competitividad (MINECO/FEDER, EU) from the Spanish Government CTQ2015-71164-P | es |
dc.format | application/pdf | es |
dc.format.extent | 13 p. | es |
dc.language.iso | eng | es |
dc.publisher | Wiley | es |
dc.relation.ispartof | Journal of Biomedical Materials Research part A, March, 1-13. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Collagen | es |
dc.subject | Electrospinning | es |
dc.subject | PCL | es |
dc.subject | Scaffolds | es |
dc.subject | Skeletal muscle cells | es |
dc.title | Fabrication of hybrid scaffolds obtained from combinations of PCL with gelatin or collagen via electrospinning for skeletal muscle tissue engineering | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Ingeniería Química | es |
dc.relation.publisherversion | https://onlinelibrary.wiley.com/doi/full/10.1002/jbm.a.37156 | es |
dc.identifier.doi | 10.1002/jbm.a.37156 | es |
dc.contributor.group | Universidad de Sevilla. TEP229: Tecnología y Diseño de Productos Multicomponentes | es |
dc.journaltitle | Journal of Biomedical Materials Research part A | es |
dc.publication.issue | March | es |
dc.publication.initialPage | 1 | es |
dc.publication.endPage | 13 | es |