dc.creator | Kang, Eunju | es |
dc.creator | Wu, Jun | es |
dc.creator | Martí Gutiérrez, Nuria | es |
dc.creator | Koski, Amy | es |
dc.creator | Tippner-Hedges, Rebecca | es |
dc.creator | Agaronyan, Karen | es |
dc.creator | Platero-Luengo, Aida | es |
dc.date.accessioned | 2021-04-22T11:25:18Z | |
dc.date.available | 2021-04-22T11:25:18Z | |
dc.date.issued | 2016 | |
dc.identifier.citation | Kang, E., Wu, J., Martí Gutiérrez, N., Koski, A., Tippner-Hedges, R., Agaronyan, K. y Platero-Luengo, A. (2016). Mitochondrial replacement in human oocytes carrying pathogenic mitochondrial DNA mutations. NATURE, 540 (7632), 270-275. | |
dc.identifier.issn | 0028-0836 | es |
dc.identifier.issn | 1476-4687(electrónico) | es |
dc.identifier.uri | https://hdl.handle.net/11441/107580 | |
dc.description | Erratum in
• Author Correction: Mitochondrial replacement in human oocytes carrying pathogenic mitochondrial DNA mutations.
Kang E, Wu J, Gutierrez NM, Koski A, Tippner-Hedges R, Agaronyan K, Platero-Luengo A, Martinez-Redondo P, Ma H, Lee Y, Hayama T, Van Dyken C, Wang X, Luo S, Ahmed R, Li Y, Ji D, Kayali R, Cinnioglu C, Olson S, Jensen J, Battaglia D, Lee D, Wu D, Huang T, Wolf DP, Temiakov D, Belmonte JCI, Amato P, Mitalipov S.Nature. 2019 Mar;567(7747):E5-E9. doi: 10.1038/s41586-019-0876-1.PMID: 30814738 | es |
dc.description.abstract | Maternally inherited mitochondrial (mt)DNA mutations can cause fatal or severely debilitating syndromes in children1,2,3, with disease severity dependent on the specific gene mutation and the ratio of mutant to wild-type mtDNA (heteroplasmy) in each cell and tissue4. Pathogenic mtDNA mutations are relatively common, with an estimated 778 affected children born each year in the United States5. Mitochondrial replacement therapies or techniques (MRT) circumventing mother–to–child mtDNA disease transmission involve replacement of oocyte maternal mtDNA6,7,8. Here we report MRT outcomes in several families with common mtDNA syndromes. The mother’s oocytes were of normal quality and mutation levels correlated with those in existing children. Efficient replacement of oocyte mutant mtDNA was performed by spindle transfer8, resulting in embryos containing >99% donor mtDNA. Donor mtDNA was stably maintained in embryonic stem cells (ES cells) derived from most embryos. However, some ES cell lines demonstrated gradual loss of donor mtDNA and reversal to the maternal haplotype. In evaluating donor–to–maternal mtDNA interactions, it seems that compatibility relates to mtDNA replication efficiency rather than to mismatch or oxidative phosphorylation dysfunction. We identify a polymorphism within the conserved sequence box II region of the D-loop as a plausible cause of preferential replication of specific mtDNA haplotypes. In addition, some haplotypes confer proliferative and growth advantages to cells. Hence, we propose a matching paradigm for selecting compatible donor mtDNA for MRT. | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | Nature Publishing Group | es |
dc.relation.ispartof | NATURE, 540 (7632), 270-275. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Mitochondrial replacement in human oocytes carrying pathogenic mitochondrial DNA mutations | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Fisiología Médica y Biofísica | es |
dc.identifier.doi | 10.1038/nature20592 | es |
dc.journaltitle | NATURE | es |
dc.publication.volumen | 540 | es |
dc.publication.issue | 7632 | es |
dc.publication.initialPage | 270 | es |
dc.publication.endPage | 275 | es |