Capítulo de Libro
The Sister-Chromatid Exchange Assay in Human Cells
Autor/es | Tumini, Emanuela
Aguilera López, Andrés |
Departamento | Universidad de Sevilla. Departamento de Genética |
Fecha de publicación | 2020 |
Fecha de depósito | 2020-09-07 |
Publicado en |
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ISBN/ISSN | 978-1-0716-0643-8 1064-3745 1940-6029 |
Resumen | The semiconservative nature of DNA replication allows the differential labeling of sister chromatids that isthe fundamental requirement to perform the sister-chromatid exchange (SCE) assay. SCE assay is apowerful technique ... The semiconservative nature of DNA replication allows the differential labeling of sister chromatids that isthe fundamental requirement to perform the sister-chromatid exchange (SCE) assay. SCE assay is apowerful technique to visually detect the physical exchange of DNA between sister chromatids. SCEscould result as a consequence of DNA damage repair by homologous recombination (HR) during DNAreplication. Here, we provide the detailed protocol to perform the SCE assay in cultured human cells. Cellsare exposed to the thymidine analog 5-bromo-20-deoxyuridine (BrdU) during two cell cycles, resulting inthe two sister chromatids having differential incorporation of the analog. After metaphase spreads prepara-tion and further processing, SCEs are nicely visualized under the microscope. |
Cita | Tumini, E., y Aguilera López, A. (2020). The Sister-Chromatid Exchange Assay in Human Cells. En Homologous Recombination Methods and Protocols (pp. 383-393). New York: Humana |
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