Artículo
Combined use of the Ab105-2фΔCI lytic mutant phage and different antibiotics in clinical isolates of multiresistant Acinetobacter baumannii
Autor/es | Blasco, L.
Ambroa, A. López, M. Fernández García, L. Rodríguez-Baño, Jesús Cisneros, José Miguel |
Departamento | Universidad de Sevilla. Departamento de Medicina |
Fecha de publicación | 2019 |
Fecha de depósito | 2020-08-28 |
Publicado en |
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Resumen | Phage therapy is an abandoned antimicrobial therapy that has been resumed in recent years. In this study, we mutated a lysogenic phage from Acinetobacter baumannii into a lytic phage (Ab105-2phiΔCI) showing antimicrobial ... Phage therapy is an abandoned antimicrobial therapy that has been resumed in recent years. In this study, we mutated a lysogenic phage from Acinetobacter baumannii into a lytic phage (Ab105-2phiΔCI) showing antimicrobial activity against A.baumannii clinical strains(such as Ab177_GEIH-2000 which showed MICs to meropenem and imipenem of 32 μg/ml and 16 μg/ml, respectively as well as belonging to GEIH-REIPI Spanish MulticenterA. baumannii Study II 2000/2010, Umbrella Genbank Bioproject PRJNA422585).We observed in vitro, an antimicrobial synergistic effect(from 4 log to 7 log CFU/ml) with meropenem plus lytic phage in all combinations analysed(0.1, 1 and 10 MOI of Ab105-2phiΔCI mutant as well as 1/4 and 1/8 MIC of meropenem). Moreover, we had a decrease in bacterial growth of 8 log CFU/ml for the combination of imipenem at 1/4 MIC plus lytic phage(Ab105-2phiΔCI mutant) and of 4 log CFU/ml for the combination of imipenem at 1/8 MIC plus lytic phage (Ab105-2phiΔCI mutant) in both MOI 1 and 10.These results were confirmed in in vivo(G. mellonella) obtaining a higher effectiveness in thecombination of imipenem and Ab105-2phiΔCI mutant(P<0.05). This approach could help to reducethe emergence of phage resistant bacteria and restore sensitivity to the antibiotics when used tocombat multiresistant strains of Acinetobacter baumannii. |
Cita | Blasco, L., Ambroa, A., López, M., Fernández García, L., Rodríguez Baño, J. y Cisneros, J.M. (2019). Combined use of the Ab105-2фΔCI lytic mutant phage and different antibiotics in clinical isolates of multiresistant Acinetobacter baumannii. Microorganisms, 7 (556), 1-12. |
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