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A functional RNase P protein subunit of bacterial origin in some eukaryotes

 

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Opened Access A functional RNase P protein subunit of bacterial origin in some eukaryotes
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Author: Lai, Lien B.
Bernal Bayard, Pilar
Mohannath, Gireesha
Lai, Stella M.
Gopalan, Venkat
Vioque Peña, Agustín
Department: Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular
Date: 2011
Published in: Molecular Genetics and Genomics, 286 (5-6), 359-369.
Document type: Article
Abstract: RNase P catalyzes 5′-maturation of tRNAs. While bacterial RNase P comprises an RNA catalyst and a protein cofactor, the eukaryotic (nuclear) variant contains an RNA and up to ten proteins, all unrelated to the bacterial protein. Unexpectedly, a nuclear-encoded bacterial RNase P protein (RPP) homolog is found in several prasinophyte algae including Ostreococcus tauri. We demonstrate that recombinant O. tauri RPP can functionally reconstitute with bacterial RNase P RNAs (RPRs) but not with O. tauri organellar RPRs, despite the latter’s presumed bacterial origins. We also show that O. tauri PRORP, a homolog of Arabidopsis PRORP-1, displays tRNA 5′-processing activity in vitro. We discuss the implications of the striking diversity of RNase P in O. tauri, the smallest known free-living eukaryote.
Cite: Lai, L.B., Bernal Bayard, P., Mohannath, G., Lai, S.M., Gopalan, V. y Vioque Peña, A. (2011). A functional RNase P protein subunit of bacterial origin in some eukaryotes. Molecular Genetics and Genomics, 286 (5-6), 359-369.
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URI: https://hdl.handle.net/11441/84574

DOI: 10.1007/s00438-011-0651-y

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