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Artículo
Human CtIP Mediates Cell Cycle Control of DNA End Resection and Double Strand Break Repair
dc.creator | Huertas Sánchez, Pablo | es |
dc.creator | Jackson, Stephen P. | es |
dc.date.accessioned | 2019-03-20T12:53:43Z | |
dc.date.available | 2019-03-20T12:53:43Z | |
dc.date.issued | 2009 | |
dc.identifier.citation | Huertas Sánchez, P. y Jackson, .P. (2009). Human CtIP Mediates Cell Cycle Control of DNA End Resection and Double Strand Break Repair. Journal of Biological Chemistry, 284 (14), 9558-9565. | |
dc.identifier.issn | 1083-351X | es |
dc.identifier.uri | https://hdl.handle.net/11441/84480 | |
dc.description.abstract | In G0 and G1, DNA double strand breaks are repaired by nonhomologous end joining, whereas in S and G2, they are also repaired by homologous recombination. The human CtIP protein controls double strand break (DSB) resection, an event that occurs effectively only in S/G2 and that promotes homologous recombination but not non-homologous end joining. Here, we mutate a highly conserved cyclin-dependent kinase (CDK) target motif in CtIP and reveal that mutating Thr-847 to Ala impairs resection, whereas mutating it to Glu to mimic constitutive phosphorylation does not. Moreover, we show that unlike cells expressing wild-type CtIP, cells expressing the Thr-to-Glu mutant resect DSBs even after CDK inhibition. Finally, we establish that Thr-847 mutations to either Ala or Glu affect DSB repair efficiency, cause hypersensitivity toward DSB-generating agents, and affect the frequency and nature of radiation-induced chromosomal rearrangements. These results suggest that CDKmediated control of resection in human cells operates by mechanisms similar to those recently established in yeast. | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | American Society for Biochemistry and Molecular Biology | es |
dc.relation.ispartof | Journal of Biological Chemistry, 284 (14), 9558-9565. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Human CtIP Mediates Cell Cycle Control of DNA End Resection and Double Strand Break Repair | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Genética | es |
dc.relation.publisherversion | http://dx.doi.org/10.1074/jbc.M808906200 | es |
dc.identifier.doi | 10.1074/jbc.M808906200 | es |
idus.format.extent | 7 p. | es |
dc.journaltitle | Journal of Biological Chemistry | es |
dc.publication.volumen | 284 | es |
dc.publication.issue | 14 | es |
dc.publication.initialPage | 9558 | es |
dc.publication.endPage | 9565 | es |
Ficheros | Tamaño | Formato | Ver | Descripción |
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pub9558.pdf | 865.6Kb | [PDF] | Ver/ | |