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Low-gluten, nontransgenic wheat engineered with CRISPR/Cas9


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Opened Access Low-gluten, nontransgenic wheat engineered with CRISPR/Cas9

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Author: Sánchez León, Susana
Gil Humanes, Javier
Ozuna Serafini, Carmen Victoria
Giménez, María J.
Sousa Martín, Carolina
Voytas, Daniel F.
Barro, Francisco
Department: Universidad de Sevilla. Departamento de Microbiología y Parasitología
Date: 2018
Published in: Plant Biotechnology Journal, 16 (4), 902-910.
Document type: Article
Abstract: Coeliac disease is an autoimmune disorder triggered in genetically predisposed individuals by the ingestion of gluten proteins from wheat, barley and rye. The a-gliadin gene family of wheat contains four highly stimulatory peptides, of which the 33-mer is the main immunodominant peptide in patients with coeliac. We designed two sgRNAs to target a conserved region adjacent to the coding sequence for the 33-mer in the a-gliadin genes. Twenty-one mutant lines were generated, all showing strong reduction in a-gliadins. Up to 35 different genes were mutated in one of the lines of the 45 different genes identified in the wild type, while immunoreactivity was reduced by 85%. Transgene-free lines were identified, and no off-target mutations have been detected in any of the potential targets. The low-gluten, transgene-free wheat lines described here could be used to produce low-gluten foodstuff and serve as source material to introgress this trait into elite wheat varieties.
Cite: Sánchez León, S., Gil Humanes, J., Ozuna, C.V., Giménez, M.J., Sousa Martín, C., Voytas, D.F. y Barro, F. (2018). Low-gluten, nontransgenic wheat engineered with CRISPR/Cas9. Plant Biotechnology Journal, 16 (4), 902-910.
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DOI: 10.1111/pbi.12837

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