dc.creator | Sánchez León, Susana | es |
dc.creator | Gil Humanes, Javier | es |
dc.creator | Ozuna Serafini, Carmen Victoria | es |
dc.creator | Giménez, María J. | es |
dc.creator | Sousa Martín, Carolina | es |
dc.creator | Voytas, Daniel F. | es |
dc.creator | Barro, Francisco | es |
dc.date.accessioned | 2018-10-30T13:09:48Z | |
dc.date.available | 2018-10-30T13:09:48Z | |
dc.date.issued | 2018 | |
dc.identifier.citation | Sánchez León, S., Gil Humanes, J., Ozuna, C.V., Giménez, M.J., Sousa Martín, C., Voytas, D.F. y Barro, F. (2018). Low-gluten, nontransgenic wheat engineered with CRISPR/Cas9. Plant Biotechnology Journal, 16 (4), 902-910. | |
dc.identifier.issn | 1467-7644 (impreso) | es |
dc.identifier.issn | 1467-7652 (electrónico) | es |
dc.identifier.uri | https://hdl.handle.net/11441/79719 | |
dc.description.abstract | Coeliac disease is an autoimmune disorder triggered in genetically predisposed individuals by the
ingestion of gluten proteins from wheat, barley and rye. The a-gliadin gene family of wheat
contains four highly stimulatory peptides, of which the 33-mer is the main immunodominant
peptide in patients with coeliac. We designed two sgRNAs to target a conserved region adjacent
to the coding sequence for the 33-mer in the a-gliadin genes. Twenty-one mutant lines were
generated, all showing strong reduction in a-gliadins. Up to 35 different genes were mutated in
one of the lines of the 45 different genes identified in the wild type, while immunoreactivity was
reduced by 85%. Transgene-free lines were identified, and no off-target mutations have been
detected in any of the potential targets. The low-gluten, transgene-free wheat lines described
here could be used to produce low-gluten foodstuff and serve as source material to introgress
this trait into elite wheat varieties. | es |
dc.description.sponsorship | Ministerio de Economía y Competitividad AGL2013-48946-C3-1-R ; AGL2016-80566-P | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | Wiley Open Access | es |
dc.relation.ispartof | Plant Biotechnology Journal, 16 (4), 902-910. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Coeliac disease | es |
dc.subject | A-gliadins | es |
dc.subject | CRISPR/Cas9 | es |
dc.title | Low-gluten, nontransgenic wheat engineered with CRISPR/Cas9 | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Microbiología y Parasitología | es |
dc.relation.projectID | AGL2013-48946-C3-1-R | es |
dc.relation.projectID | AGL2016-80566-P | es |
dc.relation.publisherversion | http://dx.doi.org/10.1111/pbi.12837 | es |
dc.identifier.doi | 10.1111/pbi.12837 | es |
idus.format.extent | 9 p. | es |
dc.journaltitle | Plant Biotechnology Journal | es |
dc.publication.volumen | 16 | es |
dc.publication.issue | 4 | es |
dc.publication.initialPage | 902 | es |
dc.publication.endPage | 910 | es |
dc.contributor.funder | Ministerio de Economía y Competitividad (MINECO). España | |