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Induction of petite yeast mutants by membrane-active agents

 

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dc.creator Jiménez Martínez, Juan es
dc.creator Longo, Elisa es
dc.creator Benítez, Tahía es
dc.date.accessioned 2018-10-03T08:17:41Z
dc.date.available 2018-10-03T08:17:41Z
dc.date.issued 1988
dc.identifier.citation Jiménez Martínez, J., Longo, E. y Benítez, T. (1988). Induction of petite yeast mutants by membrane-active agents. Applied and Environmental Microbiology, 54 (12), 3126-3132.
dc.identifier.issn 0099-2240 es
dc.identifier.uri https://hdl.handle.net/11441/79037
dc.description.abstract Ethanol proved to be a strong mutagenic agent of Saccharomyces mitochondrial DNA. Other active membrane solvents, such as tert-butanol, isopropanol, and sodium dodecyl sulfate, also turned out to be powerful petite mutation [rho-] inducers. Mutants defective in ergosterol synthesis (erg mutants) showed an extremely high frequency of spontaneous petite cells, suggesting that mitochondrial membrane alterations that were caused either by changes in its composition, as in the erg mutants, or by the effects of organic solvents resulted in an increase in the proportion of petite mutants. Wine yeast strains were generally more tolerant to the mutagenic effects of alcohols on mitochondrial DNA and more sensitive to the effect of sodium dodecyl sulfate than laboratory strains. However, resistance to petite mutation formation in laboratory strains was increased by mitochondrial transfer from alcohol-tolerant wine yeasts. Hence, the stability of the [rho+] mitochondrial DNA in either the presence or absence of solvents depends in part on the nature of the mitochondrial DNA itself. The low frequency of petite mutants found in wine yeast-laboratory yeast hybrids and the fact that the high frequency of petite mutants of a particular wine spore segregated meiotically indicated that many nuclear genes also play an important role in the mitochondrial genome in both the presence and absence of membrane solvents. es
dc.format application/pdf es
dc.language.iso eng es
dc.publisher American Society for Microbiology es
dc.relation.ispartof Applied and Environmental Microbiology, 54 (12), 3126-3132.
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 Internacional *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/ *
dc.title Induction of petite yeast mutants by membrane-active agents es
dc.type info:eu-repo/semantics/article es
dc.type.version info:eu-repo/semantics/publishedVersion es
dc.rights.accessrights info:eu-repo/semantics/openAccess es
dc.contributor.affiliation Universidad de Sevilla. Departamento de Genética es
idus.format.extent 6 p. es
dc.journaltitle Applied and Environmental Microbiology es
dc.publication.volumen 54 es
dc.publication.issue 12 es
dc.publication.initialPage 3126 es
dc.publication.endPage 3132 es
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