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Voltammetric study of the adsorbed thermophilic plastocyanin from Phormidium laminosum up to 90 °c

Opened Access Voltammetric study of the adsorbed thermophilic plastocyanin from Phormidium laminosum up to 90 °c

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Autor: Olloqui Sariego, José Luis
Frutos Beltrán, Estrella
Roldán González, Emilio
Rosa Acosta, Miguel Ángel de la
Calvente Pacheco, Juan José
Díaz Quintana, Antonio Jesús
Andreu Fondacabe, Rafael Jesús
Departamento: Universidad de Sevilla. Departamento de Química Física
Fecha: 2012
Publicado en: Electrochemistry Communications, 19, 105-107.
Tipo de documento: Artículo
Resumen: Redox thermodynamics and kinetics of plastocyanin from Phormidium laminosum, and of azurin from Pseudonomas aeruginosa, have been investigated as a function of temperature by protein film voltammetry. To this purpose, both proteins have been physisorbed on a pyrolytic graphite edge electrode. A pronounced negative shift of the plastocyanin standard potential, compared to a slight shift in the case of azurin, has been found upon increasing the temperature. Hence, significant conformational and/or solvation changes accompany the redox conversion of plastocyanin. Lower electron transfer rate constants (by c.a. one order of magnitude) and higher activation enthalpies have been found for plastocyanin as compared to azurin. The voltammetric response of azurin vanishes irreversibly at temperatures close to 60 °C, whereas the redox properties of plastocyanin remain unaltered, except for some loss of electroactive protein, after heating the electrode at temperatures as high as 90 °C.
Cita: Olloqui Sariego, J.L., Frutos Beltrán, E., Roldán González, E., Rosa Acosta, M.Á.d.l., Calvente Pacheco, J.J., Díaz Quintana, A.J. y Andreu Fondacabe, R.J. (2012). Voltammetric study of the adsorbed thermophilic plastocyanin from Phormidium laminosum up to 90 °c. Electrochemistry Communications, 19, 105-107.
Tamaño: 320.4Kb
Formato: PDF

URI: https://hdl.handle.net/11441/71041

DOI: 10.1016/j.elecom.2012.03.012

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