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Characterization of NO-Induced Nitrosative Status in Human Placenta from Pregnant Women with Gestational Diabetes Mellitus

 

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dc.creator Visiedo, Francisco es
dc.creator Santos Rosendo, Celeste es
dc.creator Mateos Bernal, Rosa M. es
dc.creator Gil Sánchez, María del Mar es
dc.creator Aguilar Diosdado, Manuel es
dc.date.accessioned 2018-02-23T09:40:37Z
dc.date.available 2018-02-23T09:40:37Z
dc.date.issued 2017
dc.identifier.citation Visiedo, F., Santos Rosendo, ., Mateos Bernal, .M., Gil Sánchez, M.d.M. y Aguilar Diosdado, M. (2017). Characterization of NO-Induced Nitrosative Status in Human Placenta from Pregnant Women with Gestational Diabetes Mellitus. Oxidative Medicine and Cellular Longevity, 5629341, 1-10.
dc.identifier.issn 1942-0994 es
dc.identifier.uri https://hdl.handle.net/11441/70545
dc.description.abstract Dysregulation of NO production is implicated in pregnancy-related diseases, including gestational diabetes mellitus (GDM). The role of NO and its placental targets in GDM pregnancies has yet to be determined. S-Nitrosylation is the NO-derived posttranslational protein modification that can modulate biological functions by forming NO-derived complexes with longer half-life, termed S-nitrosothiol (SNO). Our aim was to examine the presence of endogenous S-nitrosylated proteins in cysteine residues in relation to antioxidant defense, apoptosis, and cellular signal transduction in placental tissue from control (n = 8) and GDM (n = 8) pregnancies. S-Nitrosylation was measured using the biotin-switch assay, while the expression and protein activity were assessed by immunoblotting and colorimetric methods, respectively. Results indicated that catalase and peroxiredoxin nitrosylation levels were greater in GDM placentas, and that was accompanied by reduced catalase activity. S-Nitrosylation of ERK1/2 and AKT was increased in GDM placentas, and their activities were inhibited. Activities of caspase-3 and caspase-9 were increased, with the latter also showing diminished nitrosylation levels. These findings suggest that S-nitrosylation is a little-known, but critical, mechanism by which NO directly modulates key placental proteins in women with GDM and, as a consequence, maternal and fetal anomalies during pregnancy can occur. es
dc.description.sponsorship España Ministerio de Educación y Ciencia BMC2003-07072-C03-01 es
dc.description.sponsorship Junta de Andalucía CVI271 es
dc.format application/pdf es
dc.language.iso eng es
dc.publisher Hindawi Publishing Corporation es
dc.relation.ispartof Oxidative Medicine and Cellular Longevity, 5629341, 1-10.
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 Internacional *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/ *
dc.title Characterization of NO-Induced Nitrosative Status in Human Placenta from Pregnant Women with Gestational Diabetes Mellitus es
dc.type info:eu-repo/semantics/article es
dc.type.version info:eu-repo/semantics/publishedVersion es
dc.rights.accessrights info:eu-repo/semantics/openAccess es
dc.contributor.affiliation Universidad de Sevilla. Departamento de Genética es
dc.relation.projectID BMC2003-07072-C03-01 es
dc.relation.projectID CVI271 es
dc.relation.publisherversion http://dx.doi.org/10.1155/2017/5629341 es
dc.identifier.doi 10.1155/2017/5629341 es
idus.format.extent 10 p. es
dc.journaltitle Oxidative Medicine and Cellular Longevity es
dc.publication.issue 5629341 es
dc.publication.initialPage 1 es
dc.publication.endPage 10 es
dc.contributor.funder Ministerio de Educación y Ciencia (MEC). España
dc.contributor.funder Junta de Andalucía
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