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dc.creatorJimeno González, Silviaes
dc.creatorGómez Herreros, Fernandoes
dc.creatorAlepuz, Paula M.es
dc.creatorChávez de Diego, Sebastiánes
dc.date.accessioned2018-02-22T15:05:06Z
dc.date.available2018-02-22T15:05:06Z
dc.date.issued2006
dc.identifier.citationJimeno González, S., Gómez Herreros, F., Alepuz, P.M. y Chavez de Diego, S. (2006). A gene-specific requirement for FACT during transcription is related to the chromatin organization of the transcribed region. Molecular and Cellular Biology, 26 (23), 8710-8721.
dc.identifier.issn0270-7306es
dc.identifier.urihttps://hdl.handle.net/11441/70526
dc.description.abstractThe FACT complex stimulates transcription elongation on nucleosomal templates. In vivo experiments also involve FACT in the reassembly of nucleosomes traversed by RNA polymerase II. Since several features of chromatin organization vary throughout the genome, we wondered whether FACT is equally required for all genes. We show in this study that the in vivo depletion of Spt16, one of the subunits of Saccharomyces cerevisiae FACT, strongly affects transcription of three genes, GAL1, PHO5, and Kluyveromyces lactis LAC4, which exhibit positioned nucleosomes at their transcribed regions. In contrast, showing a random nucleosome structure, YAT1 and Escherichia coli lacZ are only mildly influenced by Spt16 depletion. We also show that the effect of Spt16 depletion on GAL1 expression is suppressed by a histone mutation and that the insertion of a GAL1 fragment, which allows the positioning of two nucleosomes, at the 5′ end of YAT1 makes the resulting transcription unit sensitive to Spt16 depletion. These results indicate that FACT requirement for transcription depends on the chromatin organization of the 5′ end of the transcribed region.es
dc.description.sponsorshipMinisterio de Educación y Ciencia BMC2003-07072-C03-01, BFU 2005-08359es
dc.description.sponsorshipJunta de Andalucía CVI271es
dc.formatapplication/pdfes
dc.language.isoenges
dc.relation.ispartofMolecular and Cellular Biology, 26 (23), 8710-8721.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectDNA-Binding Proteinses
dc.subjectProtein FACTes
dc.subjectEscherichia colies
dc.titleA gene-specific requirement for FACT during transcription is related to the chromatin organization of the transcribed regiones
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Genéticaes
dc.relation.publisherversionhttp://dx.doi.org/10.1128/MCB.01129-06es
dc.identifier.doi10.1128/MCB.01129-06es
idus.format.extent12es
dc.journaltitleMolecular and Cellular Biologyes
dc.publication.volumen26es
dc.publication.issue23es
dc.publication.initialPage8710es
dc.publication.endPage8721es
dc.contributor.funderMinisterio de Educación y Ciencia (MEC). España
dc.contributor.funderJunta de Andalucía

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