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Energy-dependent accumulation of norfloxacin and porin expression in clinical isolates of Klebsiella pneumoniae and relationship to extended-spectrum β-lactamase production

Opened Access Energy-dependent accumulation of norfloxacin and porin expression in clinical isolates of Klebsiella pneumoniae and relationship to extended-spectrum β-lactamase production

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Autor: Martínez Martínez, Luis
Pascual Hernández, Álvaro
Conejo Gonzalo, Mª Carmen
García Luque, Isabel
Joyanes, Providencia
Doménech Sánchez, Antonio
Benedí, Vicente J.
Departamento: Universidad de Sevilla. Departamento de Microbiología
Fecha: 2002-12-01
Publicado en: Antimicrobial Agents and Chemotherapy, 46 (12), 3926-3932.
Tipo de documento: Artículo
Resumen: The relationships between porin deficiency, active efflux of fluoroquinolones, and extended-spectrum β-lactamase (ESBL) production were determined for 53 clinical isolates of Klebsiella pneumoniae. Thirty-two ESBL-positive strains (including 22 strains expressing porins and 10 strains lacking porins) and 21 ESBL-negative strains were evaluated. Active efflux of norfloxacin was defined as a ≥50% increase in the accumulation of norfloxacin in the presence of carbonyl cyanide m-chlorophenylhydrazone (CCCP) in comparison with the corresponding basal value in the absence of CCCP. The quinolone resistance-determining regions of both gyrA and parC from 13 strains, representing all isolates with different porin profiles and with or without active efflux, were determined. Porin loss was significantly more common among ESBL-positive strains (10 of 32 [31.2%]) than among ESBL-negative strains (0 of 2 [0%]) (P < 0.01). Active efflux was observed in 7 of 10 (70%) strains lacking porins and in 4 of...
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Cita: Martínez Martínez, L., Pascual Hernández, Á., Conejo Gonzalo, M.C., García Luque, I., Joyanes, P., Doménech Sánchez, A. y Benedí, V.J. (2002). Energy-dependent accumulation of norfloxacin and porin expression in clinical isolates of Klebsiella pneumoniae and relationship to extended-spectrum β-lactamase production. Antimicrobial Agents and Chemotherapy, 46 (12), 3926-3932.
Tamaño: 182.8Kb
Formato: PDF

URI: http://hdl.handle.net/11441/65005

DOI: 10.1128/AAC.46.12.3926-3932.2002

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