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Changes of ploidy during the Azotobacter vinelandii growth cycle

Opened Access Changes of ploidy during the Azotobacter vinelandii growth cycle
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Autor: Maldonado, Rafael
Pérez Jiménez, Juan Carlos
Casadesús Pursals, Josep
Departamento: Universidad de Sevilla. Departamento de Genética
Fecha: 1994
Publicado en: Journal of Bacteriology, 176 (13), 3911-3919.
Tipo de documento: Artículo
Resumen: The size of the Azotobacter vinelandii chromosome is ~4,700 kb, as calculated by pulsed-field electrophoretic separation of fragments digested with the rarely cutting endonucleases SpeI and SwaI. Surveys of DNA content per cell by flow cytometry indicated the existence of ploidy changes during the A. vinelandii growth cycle in rich medium. Early-exponential-phase cells have a ploidy level similar to that of Escherichia coli or Salmonella typhimurium (probably ca. four chromosomes per cell), but a continuous increase of DNA content per cell is observed during growth. Late-exponential- phase cells may contain >40 chromosomes per cell, while cells in the early stationary stage may contain >80 chromosomes per cell. In late-stationary- phase cultures, the DNA content per cell is even higher, probably over 100 chromosome equivalents per cell. A dramatic change is observed in old stationary-phase cultures, when the population of highly polyploid bacteria segregates cells with low ploidy. The...
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Cita: Maldonado, R., Pérez Jiménez, J.C. y Casadesús Pursals, J. (1994). Changes of ploidy during the Azotobacter vinelandii growth cycle. Journal of Bacteriology, 176 (13), 3911-3919.
Tamaño: 1.844Mb
Formato: PDF

URI: http://hdl.handle.net/11441/64862

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