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Uptake and intracellular activity of an optically active ofloxacin isomer in human neutrophils and tissue culture cells

Opened Access Uptake and intracellular activity of an optically active ofloxacin isomer in human neutrophils and tissue culture cells
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Autor: Pascual Hernández, Álvaro
García Luque, Isabel
Perea Pérez, Evelio José
Departamento: Universidad de Sevilla. Departamento de Microbiología
Fecha: 1990-01-01
Publicado en: Antimicrobial Agents and Chemotherapy, 34 (2), 277-280.
Tipo de documento: Artículo
Resumen: The penetration of an optically active ofloxacin isomer [(-)-ofloxacin] into human neutrophils and different tissue culture cells (HEp-2, McCoy, MDCK, and Vero) was studied and compared with that of ofloxacin by a fluorometric assay. The cellular-to-extracellular-concentration ratios (C/E) of (-)-ofloxacin were always higher than 6, significantly greater than those of ofloxacin at extracellular concentrations of 5 and 10 mg/liter. The penetration of (-)-ofloxacin and ofloxacin was doubled when neutrophils were stimulated by phorbol myristate acetate but not affected after ingestion of opsonized Staphylococcus aureus. The C/E ratios of (-)-ofloxacin and ofloxacin for different tissue culture epithelial cells and fibroblasts were lower than those of neutrophils but still higher than 2. Both compounds produced a significant reduction in viable intraphagocytic S. aureus during 3 h of exposure to antimicrobial agents. We conclude that (-)-ofloxacin appears to reach higher intracellular con...
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Cita: Pascual Hernández, Á., García Luque, I. y Perea Pérez, E.J. (1990). Uptake and intracellular activity of an optically active ofloxacin isomer in human neutrophils and tissue culture cells. Antimicrobial Agents and Chemotherapy, 34 (2), 277-280.
Tamaño: 664.5Kb
Formato: PDF

URI: http://hdl.handle.net/11441/64846

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