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Intracellular penetration and activity of BAY Y 3118 in human polymorphonuclear leukocytes

Opened Access Intracellular penetration and activity of BAY Y 3118 in human polymorphonuclear leukocytes
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Autor: García Luque, Isabel
Pascual Hernández, Álvaro
Perea Pérez, Evelio José
Departamento: Universidad de Sevilla. Departamento de Microbiología
Fecha: 1994-01-01
Publicado en: Antimicrobial Agents and Chemotherapy, 38 (10), 2426-2429.
Tipo de documento: Artículo
Resumen: The penetration of a new quinolone (BAY Y 3118) into human polymorphonuclear leukocytes (PMNs) was evaluated by a fluorometric assay. The cellular concentration-to-extracellular concentration (C/E) ratio was higher than 6.3 at extracellular concentrations ranging from 2 to 100 mg/liter. The uptake of BAY Y 3118 was rapid, reversible and nonsaturable. The intracellular penetration of BAY Y 3118 was significantly affected by environmental temperature (C/E ratio at 4°C, 5.4 ± 0.5; control, 7.5 ± 0.9; P < 0.05) and cell viability (C/E ratio in dead PMNs, 5.5 ± 0.8; control, 7.5 ± 0.9; P < 0.05), but it was not affected by metabolic inhibitors. The ingestion of opsonized zymosan or opsonized Staphylococcus aureus significantly decreased the levels of PMN-associated BAY Y 3118. Cell stimulation by a membrane activator, however, significantly increased the intracellular concentration of this quinolone. At therapeutic extracellular concentrations (0.5, 2, and 5 mg/liter), BAY Y 3118 showed in...
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Cita: García Luque, I., Pascual Hernández, Á. y Perea Pérez, E.J. (1994). Intracellular penetration and activity of BAY Y 3118 in human polymorphonuclear leukocytes. Antimicrobial Agents and Chemotherapy, 38 (10), 2426-2429.
Tamaño: 755.6Kb
Formato: PDF

URI: http://hdl.handle.net/11441/64836

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