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Defective histone supply causes condensin-dependent chromatin alterations, SAC activation and chromosome decatenation impairment

Opened Access Defective histone supply causes condensin-dependent chromatin alterations, SAC activation and chromosome decatenation impairment

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Autor: Monje Casas, Fernando
Murillo Pineda, Marina
Cabello Lobato, María José
Clemente Ruiz, Marta
Prado, Félix
Departamento: Universidad de Sevilla. Departamento de Genética
Fecha: 2014
Publicado en: Nucleic Acids Research, 42 (20), 12469-12482.
Tipo de documento: Artículo
Resumen: The structural organization of chromosomes is essential for their correct function and dynamics during the cell cycle. The assembly of DNA into chromatin provides the substrate for topoisomerases and condensins, which introduce the different levels of superhelical torsion required for DNA metabolism. In particular, Top2 and condensin are directly involved in both the resolution of precatenanes that form during replication and the formation of the intramolecular loop that detects tension at the centromeric chromatin during chromosome biorientation. Here we show that histone depletion activates the spindle assembly checkpoint (SAC) and impairs sister chromatid decatenation, leading to chromosome mis-segregation and lethality in the absence of the SAC. We demonstrate that histone depletion impairs chromosome biorientation and activates the Aurora-dependent pathway, which detects tension problems at the kinetochore. Interestingly, SAC activation is suppressed by the absence of Top2 and Sm...
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Cita: Monje Casas, F., Murillo Pineda, M., Cabello Lobato, M.J., Clemente Ruiz, M. y Prado, F. (2014). Defective histone supply causes condensin-dependent chromatin alterations, SAC activation and chromosome decatenation impairment. Nucleic Acids Research, 42 (20), 12469-12482.
Tamaño: 13.22Mb
Formato: PDF

URI: http://hdl.handle.net/11441/64160

DOI: 10.1093/nar/gku927

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