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Mechanism of coordinated synthesis of the antagonistic regulatory proteins NifL and NifA of Klebsiella pneumoniae

Opened Access Mechanism of coordinated synthesis of the antagonistic regulatory proteins NifL and NifA of Klebsiella pneumoniae

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Autor: Govantes, Fernando
Molina López, J. A.
Santero Santurino, Eduardo
Departamento: Universidad de Sevilla. Departamento de Genética
Fecha: 1996-12
Publicado en: Journal of Bacteriology, 178 (23), 6817-6823.
Tipo de documento: Artículo
Resumen: The nifLA operon of Klebsiella pneumoniae codes for the two antagonistic regulatory proteins which control expression of all other nitrogen fixation genes. NifA is a transcriptional activator, and NifL inhibits NifA. The importance of a correct NifL-NifA stoichiometry for efficient regulation of nitrogen fixation genes has been investigated by constructing a strain with an altered nifL-nifA gene dosage ratio, resulting from the integration of an extra copy of nifA. Results showed that a balanced synthesis of both gene products is essential for correct regulation. Effects of mutations provoking translation termination of nifL upstream or downstream of its natural stop codon, combined with overproduction of both proteins when the genes are transcribed and translated from signals of the φ10 gene of the phage T7, showed that, in addition to the previously reported transcriptional polarity, there is translational coupling between nifL and nifA. In spite of the apparently efficient ribosome...
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Cita: Govantes, F., Molina López, J.A. y Santero Santurino, E. (1996). Mechanism of coordinated synthesis of the antagonistic regulatory proteins NifL and NifA of Klebsiella pneumoniae. Journal of Bacteriology, 178 (23), 6817-6823.
Tamaño: 329.8Kb
Formato: PDF

URI: http://hdl.handle.net/11441/63083

DOI: 10.1128/jb.178.23.6817-6823.1996

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