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Identification of an extradiol dioxygenase involved in tetralin biodegradation: Gene sequence analysis and purification and characterization of the gene product

Opened Access Identification of an extradiol dioxygenase involved in tetralin biodegradation: Gene sequence analysis and purification and characterization of the gene product

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Autor: Andújar, Eloísa
Hernáez, María José
Kaschabek, Stefan R.
Reineke, Walter
Santero, Eduardo
Departamento: Universidad de Sevilla. Departamento de Genética
Fecha: 2000-02
Publicado en: Journal of Bacteriology, 182 (3), 789-795.
Tipo de documento: Artículo
Resumen: A genomic region involved in tetralin biodegradation was recently identified in Sphingomonas strain TFA. We have cloned and sequenced from this region a gene designated thnC, which codes for an extradiol dioxygenase required for tetralin utilization. Comparison to similar sequences allowed us to define a subfamily of 1,2-dihydroxynaphthalene extradiol dioxygenases, which comprises two clearly different groups, and to show that ThnC clusters within group 2 of this subfamily. 1,2-Dihydroxy-5,6,7,8-tetrahydronaphthalene was found to be the metabolite accumulated by a thnC insertion mutant. The ring cleavage product of this metabolite exhibited behavior typical of a hydroxymuconic semialdehyde toward pH-dependent changes and derivatization with ammonium to give a quinoline derivative. The gene product has been purified, and its biochemical properties have been studied. The enzyme is a decamer which requires Fe(II) for activity and shows high activity toward its substrate (V(max), 40.5 U m...
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Cita: Andújar, E., Hernáez, M.J., Kaschabek, S.R., Reineke, W. y Santero, E. (200-). Identification of an extradiol dioxygenase involved in tetralin biodegradation: Gene sequence analysis and purification and characterization of the gene product. Journal of Bacteriology, 182 (3), 789-795.
Tamaño: 804.6Kb
Formato: PDF

URI: http://hdl.handle.net/11441/63005

DOI: 10.1128/JB.182.3.789-795.2000

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