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Role of two NtcA-binding sites in the complex ntcA gene promoter of the heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120

Opened Access Role of two NtcA-binding sites in the complex ntcA gene promoter of the heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120

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Autor: Olmedo Verd, Elvira
Valladares Ruiz, Ana
Flores García, Enrique
Herrero Moreno, Antonia
Muro Pastor, Alicia María
Departamento: Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular
Fecha: 2008
Publicado en: Journal of Bacteriology, 190 (22), 7584-7590.
Tipo de documento: Artículo
Resumen: Anabaena sp. strain PCC 7120 is a filamentous cyanobacterium that fixes N2 in specialized cells called heterocysts, which differentiate from vegetative cells in a process that requires the nitrogen control transcription factor NtcA. 2-Oxoglutarate-stimulated binding of purified NtcA to wild-type and modified versions of the ntcA gene promoter from Anabaena sp. was analyzed by mobility shift and DNase I footprinting assays, and the role of NtcA-binding sites in the expression of the ntcA gene during heterocyst differentiation was studied in vivo by using an ntcA-gfp translational fusion and primer extension analysis. Mutation of neither of the two identified NtcA-binding sites eliminated localized expression of ntcA in proheterocysts, but mutation of both sites led to very low, nonlocalized expression.
Cita: Olmedo Verd, E., Valladares Ruiz, A., Flores García, E., Herrero, A. y Muro Pastor, A.M. (2008). Role of two NtcA-binding sites in the complex ntcA gene promoter of the heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120. Journal of Bacteriology, 190 (22), 7584-7590.
Tamaño: 1.042Mb
Formato: PDF

URI: http://hdl.handle.net/11441/62948

DOI: 10.1128/JB.00856-08

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