Article
Role of two NtcA-binding sites in the complex ntcA gene promoter of the heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120
Author/s | Olmedo Verd, Elvira de
Valladares Ruiz, Ana Flores García, Enrique Herrero Moreno, Antonia Muro Pastor, Alicia María |
Department | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular |
Publication Date | 2008 |
Deposit Date | 2017-07-24 |
Published in |
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Abstract | Anabaena sp. strain PCC 7120 is a filamentous cyanobacterium that fixes N2 in specialized cells called heterocysts, which differentiate from vegetative cells in a process that requires the nitrogen control transcription ... Anabaena sp. strain PCC 7120 is a filamentous cyanobacterium that fixes N2 in specialized cells called heterocysts, which differentiate from vegetative cells in a process that requires the nitrogen control transcription factor NtcA. 2-Oxoglutarate-stimulated binding of purified NtcA to wild-type and modified versions of the ntcA gene promoter from Anabaena sp. was analyzed by mobility shift and DNase I footprinting assays, and the role of NtcA-binding sites in the expression of the ntcA gene during heterocyst differentiation was studied in vivo by using an ntcA-gfp translational fusion and primer extension analysis. Mutation of neither of the two identified NtcA-binding sites eliminated localized expression of ntcA in proheterocysts, but mutation of both sites led to very low, nonlocalized expression. |
Funding agencies | Ministerio de Educación y Ciencia (MEC). España |
Project ID. | BFU2004-00872
BFU2007-60457 |
Citation | Olmedo Verd, E., Valladares Ruiz, A., Flores García, E., Herrero, A. y Muro Pastor, A.M. (2008). Role of two NtcA-binding sites in the complex ntcA gene promoter of the heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120. Journal of Bacteriology, 190 (22), 7584-7590. |
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