Autor: |
Núñez Hernández, Cristina
Alonso, Ana Pucciarelli, María Graciela Casadesús Pursals, Josep García del Portillo, Francisco |
Departamento: | Universidad de Sevilla. Departamento de Genética |
Fecha: | 2014-01 |
Publicado en: | Infection and Immunity, 82 (1), 221-232. |
Tipo de documento: | Artículo |
Resumen: |
Salmonella enterica uses effector proteins delivered by type III secretion systems (TTSS) to colonize eukaryotic cells. Recent in vivo studies have shown that intracellular bacteria activate the TTSS encoded by Salmonella pathogenicity island-2 (SPI-2) to restrain growth inside phagocytes. Growth attenuation is also observed in vivo in bacteria colonizing nonphagocytic stromal cells of the intestinal lamina propria and in cultured fibroblasts. SPI-2 is required for survival of nongrowing bacteria persisting inside fibroblasts, but its induction mode and the effectors involved remain unknown. Here, we show that nongrowing dormant intracellular bacteria use the two-component system OmpR-EnvZ to induce SPI-2 expression and the PhoP-PhoQ system to regulate the time at which induction takes place, 2 h postentry. Dormant bacteria were shown to discriminate the usage of SPI-2 effectors. Among the effectors tested, SseF, SseG, and SseJ were required for survival, while others, such as SifA an... [Ver más] |
Cita: | Núñez Hernández, C., Alonso, A., Pucciarelli, M.G., Casadesús Pursals, J. y García del Portillo, F. (2014). Dormant intracellular salmonella enterica serovar typhimurium discriminates among salmonella pathogenicity island 2 effectors to persist inside fibroblasts. Infection and Immunity, 82 (1), 221-232. |
URI: http://hdl.handle.net/11441/62640
DOI: 10.1128/IAI.01304-13
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