dc.creator | Chávez de Diego, Sebastián | es |
dc.creator | García Rubio, María Luisa | es |
dc.creator | Prado Velasco, José Félix | es |
dc.creator | Aguilera López, Andrés | es |
dc.date.accessioned | 2017-07-05T12:29:28Z | |
dc.date.available | 2017-07-05T12:29:28Z | |
dc.date.issued | 2001 | |
dc.identifier.citation | Chávez de Diego, S., García Rubio, M.L., Prado Velasco, J.F. y Aguilera López, A. (2001). Hpr1 is preferentially required for transcription of either long or G+C-rich DNA sequences in Saccharomyces cerevisiae. Molecular and Cellular Biology, 21 (20), 7054-7064. | |
dc.identifier.issn | 0270-7306 | es |
dc.identifier.uri | http://hdl.handle.net/11441/62052 | |
dc.description.abstract | Hpr1 forms, together with Tho2, Mft1, and Thp2, the THO complex, which controls transcription elongation
and genome stability in Saccharomyces cerevisiae. Mutations in genes encoding the THO complex confer strong
transcription-impairment and hyperrecombination phenotypes in the bacterial lacZ gene. In this work we
demonstrate that Hpr1 is a factor required for transcription of long as well as G C-rich DNA sequences. Using
different lacZ segments fused to the GAL1 promoter, we show that the negative effect of lacZ sequences on
transcription depends on their distance from the promoter. In parallel, we show that transcription of either a
long LYS2 fragment or the S. cerevisiae YAT1 G C-rich open reading frame fused to the GAL1 promoter is
severely impaired in hpr1 mutants, whereas transcription of LAC4, the Kluyveromyces lactis ortholog of lacZ but
with a lower G C content, is only slightly affected. The hyperrecombination behavior of the DNA sequences
studied is consistent with the transcriptional defects observed in hpr1 cells. These results indicate that both
length and G C content are important elements influencing transcription in vivo. We discuss their relevance
for the understanding of the functional role of Hpr1 and, by extension, the THO complex. | es |
dc.description.sponsorship | Ministerio de Ciencia y Cultura PB96-1350 | es |
dc.description.sponsorship | Human Frontier Science Program RG0075/1999-M | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | American Society for Microbiology | es |
dc.relation.ispartof | Molecular and Cellular Biology, 21 (20), 7054-7064. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Hpr1 is preferentially required for transcription of either long or G+C-rich DNA sequences in Saccharomyces cerevisiae | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Genética | es |
dc.relation.projectID | PB96-1350 | es |
dc.relation.projectID | RG0075/1999-M | es |
dc.relation.publisherversion | http://dx.doi.org/10.1128/MCB.21.20.7054–7064.2001 | es |
dc.identifier.doi | 10.1128/MCB.21.20.7054–7064.2001 | es |
idus.format.extent | 11 p. | es |
dc.journaltitle | Molecular and Cellular Biology | es |
dc.publication.volumen | 21 | es |
dc.publication.issue | 20 | es |
dc.publication.initialPage | 7054 | es |
dc.publication.endPage | 7064 | es |
dc.identifier.sisius | 6655186 | es |
dc.contributor.funder | Ministerio de Educación y Cultura (MEC). España | |
dc.contributor.funder | Human Frontier Science Program (HFSP) | |