dc.creator | Gómez González, Belén | es |
dc.creator | Felipe Abrio, Irene | es |
dc.creator | Aguilera López, Andrés | es |
dc.date.accessioned | 2017-07-04T10:38:24Z | |
dc.date.available | 2017-07-04T10:38:24Z | |
dc.date.issued | 2009 | |
dc.identifier.citation | Gómez González, B., Felipe Abrio, I. y Aguilera López, A. (2009). The S-phase checkpoint is required to respond to R-loops accumulated in THO mutants. Molecular and Cellular Biology, 29 (19), 5203-5213. | |
dc.identifier.issn | 0270-7306 | es |
dc.identifier.uri | http://hdl.handle.net/11441/61932 | |
dc.description.abstract | Cotranscriptional R-loops are formed in yeast mutants of the THO complex, which functions at the interface
between transcription and mRNA export. Despite the relevance of R-loops in transcription-associated recombination,
the mechanisms by which they trigger recombination are still elusive. In order to understand how
R-loops compromise genome stability, we have analyzed the genetic interaction of THO with 26 genes involved
in replication, S-phase checkpoint, DNA repair, and chromatin remodeling. We found a synthetic growth defect
in double null mutants of THO and S-phase checkpoint factors, such as the replication factor C- and
PCNA-like complexes. Under replicative stress, R-loop-forming THO null mutants require functional S-phase
checkpoint functions but not double-strand-break repair functions for survival. Furthermore, R-loop-forming
hpr1 mutants display replication fork progression impairment at actively transcribed chromosomal regions
and trigger Rad53 phosphorylation. We conclude that R-loop-mediated DNA damage activates the S-phase
checkpoint, which is required for the cell survival of THO mutants under replicative stress. In light of these
results, we propose a model in which R-loop-mediated recombination is explained by template switching | es |
dc.description.sponsorship | Ministerio de Ciencia e Innovación BFU2006-05260 CDS2007-0015 | es |
dc.description.sponsorship | Junta de Andalucía CVI-624 BIO-102 | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | American Society for Microbiology | es |
dc.relation.ispartof | Molecular and Cellular Biology, 29 (19), 5203-5213. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | The S-phase checkpoint is required to respond to R-loops accumulated in THO mutants | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Genética | es |
dc.relation.projectID | BFU2006-05260 | es |
dc.relation.projectID | CDS2007-0015 | es |
dc.relation.projectID | CVI-624 | es |
dc.relation.projectID | BIO-102 | es |
dc.relation.publisherversion | 10.1128/MCB.00402-09 | es |
dc.identifier.doi | 10.1128/MCB.00402-09 | es |
idus.format.extent | 10 p. | es |
dc.journaltitle | Molecular and Cellular Biology | es |
dc.publication.volumen | 29 | es |
dc.publication.issue | 19 | es |
dc.publication.initialPage | 5203 | es |
dc.publication.endPage | 5213 | es |
dc.contributor.funder | Ministerio de Ciencia e Innovación (MICIN). España | |
dc.contributor.funder | Junta de Andalucía | |