dc.creator | Romero Gómez, Manuel | es |
dc.creator | Jover Cobos, María | es |
dc.creator | Díaz Gómez, Daniel | es |
dc.creator | Collantes de Terán, Laura | es |
dc.creator | Rodrigo, Regina | es |
dc.creator | Camacho Benítez, Inés | es |
dc.creator | Echevarría, Miriam | es |
dc.creator | Felipo, Vicente | es |
dc.creator | Bautista Palomas, Juan Dionisio | es |
dc.date.accessioned | 2017-06-13T09:44:30Z | |
dc.date.available | 2017-06-13T09:44:30Z | |
dc.date.issued | 2006-04-21 | |
dc.identifier.citation | Romero Gómez, M., Jover Cobos, M., Díaz Gómez, D., Collantes de Terán, L., Rodrigo, R., Camacho Benítez, I.,...,Bautista Palomas, J.D. (2006). Phosphate-activated glutaminase activity is enhanced in brain, intestine and kidneys of rats following portacaval anastomosis. World Journal of Gastroenterology, 12 (15), 2406-2411. | |
dc.identifier.issn | 10079327 | es |
dc.identifier.uri | http://hdl.handle.net/11441/61241 | |
dc.description.abstract | AIM: To assess whether portacaval anastomosis (PCA) in rats affects the protein expression and/or activity of glutaminase in kidneys, intestines and in three brain areas of cortex, basal ganglia and cerebellum and to explain the neurological alterations found in hepatic encephalopathy (HE). METHODS: Sixteen male Wistar rats weighing 250-350 gwere grouped into sham-operation control (n = 8) or portacaval shunt (n = 8). Twenty-eight days after the
procedure, the animals were sacrificed. The duodenum, kidney and brain were removed, homogenised and mitochondria were isolated. Ammonia was measured in brain and blood. Phosphate-activated glutaminase (PAG) activity was determined by measuring ammonia production following incubation for one hour at 37 with O-phthalaldehyde (OPA) and specific activity expressed in units per gram of protein (μkat/g of protein). Protein expression was measured by immunoblotting.
RESULTS: Duodenal and kidney PAG activities together with protein content were significantly higher in PCA group than in control or sham-operated rats (duodenum PAG activity was 976.95±268.87 μkat/g of protein in PCA rats vs 429.19±126.92 μkat/g of protein in shamoperated rats; kidneys PAG activity was 1259.18 ± 228.79 μkat/g protein in PCA rats vs 669.67± 400.8 μkat/g of protein in controls, P < 0.05; duodenal protein content:
173% in PCA vs sham-operated rats; in kidneys the content of protein was 152% in PCA vs sham-operated rats). PAG activity and protein expression in PCA rats were higher in cortex and basal ganglia than those in shamoperated rats (cortex: 6646.6 ± 1870.4 μkat/g of protein
vs 3573.8 ± 2037.4 μkat/g of protein in control rats, P < 0.01; basal ganglia, PAG activity was 3657.3 ± 1469.6 μkat/g of protein in PCA rats vs 2271.2 ± 384 μkat/g of protein in sham operated rats, P < 0.05; In the cerebellum, the PAG activity was 2471.6 ± 701.4 μkat/g of
protein vs 1452.9 ± 567.8 μkat/g of protein in the PCA and sham rats, respectively, P < 0.05; content of protein:cerebral cortex: 162% ± 40% vs 100% ± 26%, P < 0.009;and basal gangl ia: 140% ± 39% vs 100% ± 14%,P < 0.05; but not in cerebel lum: 100% ± 25% vs 100% ± 16%, P = ns).
CONCLUSION: Increased PAG activity in kidney and duodenum could contribute significantly to the hyperammonaemia in PCA rats, animal model of encephalopathy.
PAG is increased in non-synaptic mitochondria from the cortex and basal ganglia and could be implicated in the pathogenesis of hepatic encephalopathy. Therefore, PAG could be a possible target for the treatment of HE orliver dysfunction. | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | World Journal of Gastroenterology | es |
dc.relation.ispartof | World Journal of Gastroenterology, 12 (15), 2406-2411. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Hyperammonaemia | es |
dc.subject | Minimal hepat ic encephalopathy | es |
dc.subject | Glutamine | es |
dc.subject | Protein content | es |
dc.subject | Shamoperated | es |
dc.title | Phosphate-activated glutaminase activity is enhanced in brain, intestine and kidneys of rats following portacaval anastomosis | es |
dc.type | info:eu-repo/semantics/article | es |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Medicina | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Cirugía | es |
dc.contributor.affiliation | Universidad de Sevilla.Instituto de Biomedicina de Sevilla (IBIS) | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Bioquímica y Biología Molecular | es |
dc.contributor.group | Grupo de Investigación: CTS-532 Unidad de Hepatologia | es |
idus.format.extent | 6 | es |
dc.journaltitle | World Journal of Gastroenterology | es |
dc.publication.volumen | 12 | es |
dc.publication.issue | 15 | es |
dc.publication.initialPage | 2406 | es |
dc.publication.endPage | 2411 | es |