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The scutellum of germinated wheat grains undergoes programmed cell death: identification of an acidic nuclease involved in nucleus dismantling

Opened Access The scutellum of germinated wheat grains undergoes programmed cell death: identification of an acidic nuclease involved in nucleus dismantling

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Autor: Domínguez, Fernando
Moreno Onorato, Francisco Javier
Cejudo Fernández, Francisco Javier
Departamento: Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular
Universidad de Sevilla. Departamento de Biología Celular
Fecha: 2012
Publicado en: Journal of Experimental Botany, 63 (15), 5475-5485.
Tipo de documento: Artículo
Resumen: Programmed cell death (PCD) is a crucial phenomenon in the life cycle of cereal grains. In germinating grains, the scutellum allows the transport of nutrients from the starchy endosperm to the growing embryo, and therefore it may be the last grain tissue to undergo PCD. Thus, the aim of this work was to analyse whether the scutellum of wheat grains undergoes PCD and to perform a morphological and biochemical analysis of this process. Scutellum cells of grains following germination showed a progressive increase of DNA fragmentation, and the TUNEL assay showed that PCD extended in an apical-to-basal gradient along the scutellum affecting epidermal and parenchymal cells. Electron-transmission microscopy revealed high cytoplasm vacuolation, altered mitochondria, and the presence of double-membrane structures, which might constitute symptoms of vacuolar cell death, whereas the nucleus appeared lobed and had an increased heterochromatin content as the most distinctive features. An acid- and...
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Cita: Domínguez, F., Moreno Onorato, F.J. y Cejudo Fernández, F.J. (2012). The scutellum of germinated wheat grains undergoes programmed cell death: identification of an acidic nuclease involved in nucleus dismantling. Journal of Experimental Botany, 63 (15), 5475-5485.
Tamaño: 2.487Mb
Formato: PDF

URI: http://hdl.handle.net/11441/60918

DOI: 10.1093/jxb/ers199

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