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dc.creatorDomínguez, Fernandoes
dc.creatorMoreno Onorato, Francisco Javieres
dc.creatorCejudo Fernández, Francisco Javieres
dc.date.accessioned2017-06-05T11:32:50Z
dc.date.available2017-06-05T11:32:50Z
dc.date.issued2012
dc.identifier.citationDomínguez, F., Moreno Onorato, F.J. y Cejudo Fernández, F.J. (2012). The scutellum of germinated wheat grains undergoes programmed cell death: identification of an acidic nuclease involved in nucleus dismantling. Journal of Experimental Botany, 63 (15), 5475-5485.
dc.identifier.issn0022-0957es
dc.identifier.urihttp://hdl.handle.net/11441/60918
dc.description.abstractProgrammed cell death (PCD) is a crucial phenomenon in the life cycle of cereal grains. In germinating grains, the scutellum allows the transport of nutrients from the starchy endosperm to the growing embryo, and therefore it may be the last grain tissue to undergo PCD. Thus, the aim of this work was to analyse whether the scutellum of wheat grains undergoes PCD and to perform a morphological and biochemical analysis of this process. Scutellum cells of grains following germination showed a progressive increase of DNA fragmentation, and the TUNEL assay showed that PCD extended in an apical-to-basal gradient along the scutellum affecting epidermal and parenchymal cells. Electron-transmission microscopy revealed high cytoplasm vacuolation, altered mitochondria, and the presence of double-membrane structures, which might constitute symptoms of vacuolar cell death, whereas the nucleus appeared lobed and had an increased heterochromatin content as the most distinctive features. An acid- and Zn2+-dependent nucleolytic activity was identified in nuclear extracts of scutellum cells undergoing PCD. This nuclease was not detected in grains imbibed in the presence of abscisic acid, which inhibited germination. This nucleolytic activity promoted DNA fragmentation in vitro on nuclei isolated from healthy cells, thus suggesting a main role in nucleus dismantling during PCDes
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherOxford University Presses
dc.relation.ispartofJournal of Experimental Botany, 63 (15), 5475-5485.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectcell deathes
dc.subjectgerminationes
dc.subjectnucleasees
dc.subjectscutellumes
dc.subjectseedes
dc.subjectTriticum aestivum (wheat)es
dc.titleThe scutellum of germinated wheat grains undergoes programmed cell death: identification of an acidic nuclease involved in nucleus dismantlinges
dc.typeinfo:eu-repo/semantics/articlees
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessrightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Moleculares
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Biología Celulares
dc.relation.publisherversionhttp://dx.doi.org/10.1093/jxb/ers199es
dc.identifier.doi10.1093/jxb/ers199es
idus.format.extent9 p.es
dc.journaltitleJournal of Experimental Botanyes
dc.publication.volumen63es
dc.publication.issue15es
dc.publication.initialPage5475es
dc.publication.endPage5485es

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