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Hydroxyurea-Stalled Replication Forks Become Progressively Inactivated and Require Two Different RAD51-Mediated Pathways for Restart and Repair

 

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Opened Access Hydroxyurea-Stalled Replication Forks Become Progressively Inactivated and Require Two Different RAD51-Mediated Pathways for Restart and Repair
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Author: Petermann, Eva
Orta Vázquez, Manuel Luis
Issaeva, Natalia
Schultz, Niklas
Helleday, Thomas
Department: Universidad de Sevilla. Departamento de Biología Celular
Date: 2010
Published in: Molecular Cell, 37 (4), 1-11.
Document type: Article
Abstract: Faithful DNA replication is essential to all life. Hydroxyurea (HU) depletes the cells of dNTPs, which initially results in stalled replication forks that, after prolonged treatment, collapse into DSBs. Here, we report that stalled replication forks are efficiently restarted in a RAD51-dependent process that does not trigger homologous recombination (HR). The XRCC3 protein, which is required for RAD51 foci formation, is also required for replication restart of HU-stalled forks, suggesting that RAD51-mediated strand invasion supports fork restart. In contrast, replication forks collapsed by prolonged replication blocks do not restart, and global replication is rescued by new origin firing. We find that RAD51-dependent HR is triggered for repair of collapsed replication forks, without apparent restart. In conclusion, our data suggest that restart of stalled replication forks and HR repair of collapsed replication forks require two distinct RAD51-mediated pathways.
Cite: Petermann, E., Orta Vázquez, M.L., Issaeva, N., Schultz, . y Helleday, T. (2010). Hydroxyurea-Stalled Replication Forks Become Progressively Inactivated and Require Two Different RAD51-Mediated Pathways for Restart and Repair. Molecular Cell, 37 (4), 1-11.
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URI: http://hdl.handle.net/11441/58958

DOI: 10.1016/j.molcel.2010.01.021

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