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dc.creatorLópez Garrido, Javieres
dc.creatorPuerta Fernández, Elenaes
dc.creatorCasadesús Pursals, Josepes
dc.date.accessioned2017-04-11T11:56:09Z
dc.date.available2017-04-11T11:56:09Z
dc.date.issued2014
dc.identifier.citationLópez Garrido, J., Puerta Fernández, E. y Casadesús Pursals, J. (2014). A eukaryotic-like 3′ untranslated region in Salmonella enterica hilD mRNA. Nucleic Acids Research, 42 (9), 5894-5906.
dc.identifier.issn0305-1048es
dc.identifier.urihttp://hdl.handle.net/11441/57479
dc.description.abstractLong 3' untranslated regions (3'UTRs) are common in eukaryotic mRNAs. In contrast, long 3'UTRs are rare in bacteria, and have not been characterized in detail. We describe a 3'UTR of 310 nucleotides in hilD mRNA, a transcript that encodes a transcriptional activator of Salmonella enterica pathogenicity island 1 (SPI-1). Deletion of the hilD 3'UTR increases the hilD mRNA level, suggesting that the hilD 3'UTR may play a role in hilD mRNA turnover. Cloning of the hilD 3'UTR downstream of the green fluorescent protein (gfp) gene decreases green fluorescent protein (GFP) activity in both Escherichia coli and S. enterica, indicating that the hilD 3'UTR can act as an independent module. S. enterica mutants lacking either ribonuclease E or polynucleotide phosphorylase contain similar amounts of hilD and hilD Δ3'UTR mRNAs, suggesting that the hilD 3'UTR is a target for hilD mRNA degradation by the degradosome. The hilD 3'UTR is also necessary for modulation of hilD and SPI-1 expression by the RNA chaperone Hfq. Overexpression of SPI-1 in the absence of the hilD 3'UTR retards Salmonella growth and causes uncontrolled invasion of epithelial cells. Based on these observations, we propose that the S. enterica hilD 3'UTR is a cis-acting element that contributes to cellular homeostasis by promoting hilD mRNA turnover.es
dc.description.sponsorshipMinisterio de Economía y Competitividad BIO2010- 15023 y CSD2008-00013es
dc.description.sponsorshipJunta de Andalucía P10-CVI-5879es
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherOxford University Presses
dc.relation.ispartofNucleic Acids Research, 42 (9), 5894-5906.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleA eukaryotic-like 3′ untranslated region in Salmonella enterica hilD mRNAes
dc.typeinfo:eu-repo/semantics/articlees
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Genéticaes
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO/BIO2010-15023es
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO/CSD2008-00013es
dc.relation.projectIDP10-CVI-5879es
dc.relation.publisherversion10.1093/nar/gku222es
dc.identifier.doihttp://dx.doi.org/10.1093/nar/gku222es
idus.format.extent19 p.es
dc.journaltitleNucleic Acids Researches
dc.publication.volumen42es
dc.publication.issue9es
dc.publication.initialPage5894es
dc.publication.endPage5906es
dc.contributor.funderMinisterio de Economía y Competitividad (MINECO). España
dc.contributor.funderJunta de Andalucía

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