dc.creator | López Saavedra, Ana | es |
dc.creator | Gómez Cabello, Daniel | es |
dc.creator | Domínguez Sánchez, María Salud | es |
dc.creator | Mejías Navarro, Fernando | es |
dc.creator | Fernández Ávila, María Jesús | es |
dc.creator | Martínez Macías, María Isabel | es |
dc.creator | Huertas Sánchez, Pablo | es |
dc.date.accessioned | 2017-03-17T11:13:41Z | |
dc.date.available | 2017-03-17T11:13:41Z | |
dc.date.issued | 2016 | |
dc.identifier.citation | López Saavedra, A., Gómez Cabello, D., Domínguez Sánchez, M.S., Mejías Navarro, F., Fernández Ávila, M.J., Martínez Macías, M.I. y Huertas Sánchez, P. (2016). A genome-wide screening uncovers the role of CCAR2 as an antagonist of DNA end resection. Nature Communications, 7 (12364), 1-14. | |
dc.identifier.issn | 2041-1723 | es |
dc.identifier.uri | http://hdl.handle.net/11441/55957 | |
dc.description.abstract | There are two major and alternative pathways to repair DNA double-strand breaks: non-homologous end-joining and homologous recombination. Here we identify and characterize novel factors involved in choosing between these pathways; in this study we took advantage of the SeeSaw Reporter, in which the repair of double-strand breaks by homology-independent or -dependent mechanisms is distinguished by the accumulation of green or red fluorescence, respectively. Using a genome-wide human esiRNA (endoribonuclease-prepared siRNA) library, we isolate genes that control the recombination/end-joining ratio. Here we report that two distinct sets of genes are involved in the control of the balance between NHEJ and HR: those that are required to facilitate recombination and those that favour NHEJ. This last category includes CCAR2/DBC1, which we show inhibits recombination by limiting the initiation and the extent of DNA end resection, thereby acting as an antagonist of CtIP. | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | Nature Publishing Group | es |
dc.relation.ispartof | Nature Communications, 7 (12364), 1-14. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | A genome-wide screening uncovers the role of CCAR2 as an antagonist of DNA end resection | es |
dc.type | info:eu-repo/semantics/article | es |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Genética | es |
dc.relation.publisherversion | http://dx.doi.org/ 10.1038/ncomms12364 | es |
dc.identifier.doi | 10.1038/ncomms12364 | es |
dc.journaltitle | Nature Communications | es |
dc.publication.volumen | 7 | es |
dc.publication.issue | 12364 | es |
dc.publication.initialPage | 1 | es |
dc.publication.endPage | 14 | es |