Repositorio de producción científica de la Universidad de Sevilla

GABAa and a-Amino-3-hydroxy-5-methylsoxazole-4-propionate receptors are differentially affected by aging in the rat hippocampus

 

Advanced Search
 

Show simple item record

dc.creator Ruano Caballero, Diego es
dc.creator Araujo, Francisco es
dc.creator Revilla Torres, María Elisa es
dc.creator Vela, Jose es
dc.creator Bergis, Olivier es
dc.creator Vitorica Ferrández, Francisco Javier es
dc.date.accessioned 2017-01-17T08:32:28Z
dc.date.available 2017-01-17T08:32:28Z
dc.date.issued 2000
dc.identifier.citation Ruano Caballero, D., Araujo, F., Revilla Torres, M.E., Vela, J., Bergis, O. y Vitorica Ferrández, F.J. (2000). GABAa and a-Amino-3-hydroxy-5-methylsoxazole-4-propionate receptors are differentially affected by aging in the rat hippocampus. Journal of Biological Chemistry, 275 (26), 19585-19593.
dc.identifier.issn 0021-9258 es
dc.identifier.uri http://hdl.handle.net/11441/52315
dc.description.abstract We have investigated the age-dependent modifications in the expression of eight different subunits of the γ-aminobutyric acid, type A (GABAA) receptor (α1, α2, α3, α5, β2, β3, γ2S, and γ2L) and all four subunits of the α-amino-3-hydroxy-5-methylsoxazole-4-propionate (AMPA) receptor (GluR1–4) in the hippocampus of 24-month-old rats. All aged hippocampi displayed a remarkable increase (aged/adult ratio, 3.53 ± 0.54) in the mRNA levels of the short version of the γ2 subunit in parallel with a similar increase in the γ2 subunit protein (aged/adult ratio, 2.90 ± 0.62). However, this increase was not observed in the mature receptor. On the other hand, the expression of the different α subunit mRNAs increased moderately with aging, displaying a heterogeneous pattern. The most frequent modification consisted in an increase in the expression of the α1 subunit mRNA (aged/adult ratio, 1.26 ± 0.18), in parallel with a similar increase on the α1 protein (aged/adult ratio, 1.27 ± 0.12) and in the α1 incorporated to the assembled GABAA receptor (tested by immunoprecipitation; aged/adult ratio, = 1.20 ± 0.10). However, in the same hippocampal samples, no major modifications were observed on the expression of the AMPA receptor subunits. As a whole, these results indicated the existence of an increased expression of the GABAA receptor subunits and a preservation of the AMPA receptor at the hippocampal formation. These modifications could reflect the existence of specific deficiencies (neuronal loss and/or deafferentiation) on the GABAergic system in the aged rats es
dc.format application/pdf es
dc.language.iso eng es
dc.publisher American Society for Biochemistry and Molecular Biology es
dc.relation.ispartof Journal of Biological Chemistry, 275 (26), 19585-19593.
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 Internacional *
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 Internacional *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/ *
dc.title GABAa and a-Amino-3-hydroxy-5-methylsoxazole-4-propionate receptors are differentially affected by aging in the rat hippocampus es
dc.type info:eu-repo/semantics/article es
dc.type.version info:eu-repo/semantics/publishedVersion es
dc.rights.accessrights info:eu-repo/semantics/openAccess es
dc.contributor.affiliation Universidad de Sevilla. Departamento de Bioquímica y Biología Molecular es
dc.identifier.doi 10.1074/jbc.M000700200 es
idus.format.extent 10 p. es
dc.journaltitle Journal of Biological Chemistry es
dc.publication.volumen 275 es
dc.publication.issue 26 es
dc.publication.initialPage 19585 es
dc.publication.endPage 19593 es
Size: 278.8Kb
Format: PDF

This item appears in the following Collection(s)

Show simple item record