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GABAa and a-Amino-3-hydroxy-5-methylsoxazole-4-propionate receptors are differentially affected by aging in the rat hippocampus
dc.creator | Ruano Caballero, Diego | es |
dc.creator | Araujo, Francisco | es |
dc.creator | Revilla Torres, María Elisa | es |
dc.creator | Vela, Jose | es |
dc.creator | Bergis, Olivier | es |
dc.creator | Vitorica Ferrández, Francisco Javier | es |
dc.date.accessioned | 2017-01-17T08:32:28Z | |
dc.date.available | 2017-01-17T08:32:28Z | |
dc.date.issued | 2000 | |
dc.identifier.citation | Ruano Caballero, D., Araujo, F., Revilla Torres, M.E., Vela, J., Bergis, O. y Vitorica Ferrández, F.J. (2000). GABAa and a-Amino-3-hydroxy-5-methylsoxazole-4-propionate receptors are differentially affected by aging in the rat hippocampus. Journal of Biological Chemistry, 275 (26), 19585-19593. | |
dc.identifier.issn | 0021-9258 | es |
dc.identifier.uri | http://hdl.handle.net/11441/52315 | |
dc.description.abstract | We have investigated the age-dependent modifications in the expression of eight different subunits of the γ-aminobutyric acid, type A (GABAA) receptor (α1, α2, α3, α5, β2, β3, γ2S, and γ2L) and all four subunits of the α-amino-3-hydroxy-5-methylsoxazole-4-propionate (AMPA) receptor (GluR1–4) in the hippocampus of 24-month-old rats. All aged hippocampi displayed a remarkable increase (aged/adult ratio, 3.53 ± 0.54) in the mRNA levels of the short version of the γ2 subunit in parallel with a similar increase in the γ2 subunit protein (aged/adult ratio, 2.90 ± 0.62). However, this increase was not observed in the mature receptor. On the other hand, the expression of the different α subunit mRNAs increased moderately with aging, displaying a heterogeneous pattern. The most frequent modification consisted in an increase in the expression of the α1 subunit mRNA (aged/adult ratio, 1.26 ± 0.18), in parallel with a similar increase on the α1 protein (aged/adult ratio, 1.27 ± 0.12) and in the α1 incorporated to the assembled GABAA receptor (tested by immunoprecipitation; aged/adult ratio, = 1.20 ± 0.10). However, in the same hippocampal samples, no major modifications were observed on the expression of the AMPA receptor subunits. As a whole, these results indicated the existence of an increased expression of the GABAA receptor subunits and a preservation of the AMPA receptor at the hippocampal formation. These modifications could reflect the existence of specific deficiencies (neuronal loss and/or deafferentiation) on the GABAergic system in the aged rats | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | American Society for Biochemistry and Molecular Biology | es |
dc.relation.ispartof | Journal of Biological Chemistry, 275 (26), 19585-19593. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | GABAa and a-Amino-3-hydroxy-5-methylsoxazole-4-propionate receptors are differentially affected by aging in the rat hippocampus | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Bioquímica y Biología Molecular | es |
dc.identifier.doi | 10.1074/jbc.M000700200 | es |
idus.format.extent | 10 p. | es |
dc.journaltitle | Journal of Biological Chemistry | es |
dc.publication.volumen | 275 | es |
dc.publication.issue | 26 | es |
dc.publication.initialPage | 19585 | es |
dc.publication.endPage | 19593 | es |