dc.creator | Rosado, Iván | es |
dc.creator | Dez, Christophe | es |
dc.creator | Lebaron, Simon | es |
dc.creator | Caizergues-Ferrer, Michèle | es |
dc.creator | Henry, Yves | es |
dc.creator | Cruz Díaz, Jesús de la | es |
dc.date.accessioned | 2016-08-08T11:03:20Z | |
dc.date.available | 2016-08-08T11:03:20Z | |
dc.date.issued | 2007 | |
dc.identifier.citation | Rosado, I., Dez, C., Lebaron, S., Caizergues-Ferrer, M., Henry, Y. y Cruz Díaz, J.d.l. (2007). Characterization of saccharomyces cerevisiae Npa2p (Urb2p) reveals a low-molecular-mass complex containing Dbp6p, Npa1p (Urb1p), Nop8p, and Rsa3p involved in early steps of 60S ribosomal subunit biogenesis. Molecular and Cellular Biology, 27 (4), 1207-1221. | |
dc.identifier.issn | 0270-7306 | es |
dc.identifier.uri | http://hdl.handle.net/11441/44284 | |
dc.description.abstract | We report the characterization of the yeast Npa2p (Urb2p) protein, which is essential for 60S ribosomal
subunit biogenesis. We identified this protein in a synthetic lethal screening with thersa3null allele. Rsa3p is
a genetic partner of the putative RNA helicase Dbp6p. Mutation or depletion of Npa2p leads to a net deficit
in 60S subunits and a decrease in the levels all 27S pre-rRNAs and mature 25S and 5.8S rRNAs. This is likely
due to instability of early pre-60S particles. Consistent with a role of Npa2p in 60S subunit biogenesis, green
fluorescent protein-tagged Npa2p localizes predominantly to the nucleolus and TAP-tagged Npa2p sediments
with large complexes in sucrose gradients and is associated mainly with 27SA2
pre-rRNA-containing preribosomal particles. In addition, we reveal a genetic synthetic interaction between Npa2p, several factors required
for early steps of 60S subunit biogenesis (Dbp6p, Dbp7p, Dbp9p, Npa1p, Nop8p, and Rsa3p), and the 60S
protein Rpl3p. Furthermore, coimmunoprecipitation and gel filtration analyses demonstrated that at least
Npa2p, Dbp6p, Npa1p, Nop8p, and Rsa3p are present together in a subcomplex of low molecular mass whose
integrity is independent of RNA. Our results support the idea that these five factors work in concert during the
early steps of 60S subunit biogenesis | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | American Society for Microbiology | es |
dc.relation.ispartof | Molecular and Cellular Biology, 27 (4), 1207-1221. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Characterization of saccharomyces cerevisiae Npa2p (Urb2p) reveals a low-molecular-mass complex containing Dbp6p, Npa1p (Urb1p), Nop8p, and Rsa3p involved in early steps of 60S ribosomal subunit biogenesis | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Genética | es |
dc.relation.publisherversion | http://mcb.asm.org/content/27/4/1207.full.pdf+html | es |
idus.format.extent | 15 p. | es |
dc.journaltitle | Molecular and Cellular Biology | es |
dc.publication.volumen | 27 | es |
dc.publication.issue | 4 | es |
dc.publication.initialPage | 1207 | es |
dc.publication.endPage | 1221 | es |
dc.identifier.idus | https://idus.us.es/xmlui/handle/11441/44284 | |