dc.creator | Rodríguez Ortiz, Luis Roberto | es |
dc.creator | Limón Mirón, María del Carmen | es |
dc.creator | Ávalos Cordero, Francisco Javier | es |
dc.date.accessioned | 2016-06-14T10:03:57Z | |
dc.date.available | 2016-06-14T10:03:57Z | |
dc.date.issued | 2009 | |
dc.identifier.citation | Rodríguez Ortiz, L.R., Limón Mirón, M.d.C. y Avalos Cordero, F.J. (2009). Regulation of carotenogenesis and secondary metabolism by nitrogen in wild-type Fusarium fujikuroi and carotenoid-overproducing mutants. Applied and Environmental Microbiology, 75 (2), 405-413. | |
dc.identifier.issn | 0099-2240 | es |
dc.identifier.uri | http://hdl.handle.net/11441/42223 | |
dc.description.abstract | The fungus
Fusarium fujikuroi
(
Gibberella fujikuroi
MP-C) produces metabolites of biotechnological interest,
such as gibberellins, bikaverins, and carotenoids. Gibberellin and bikaverin productions are induced upon
nitrogen exhaustion, while carotenoid accumulation is stimulated by light. We evaluated the effect of nitrogen
availability on carotenogenesis in comparison with bikaverin and gibberellin production in the wild type and
in carotenoid-overproducing mutants (
carS
). Nitrogen starvation increased carotenoid accumulation in all
strains tested. In
carS
strains, gibberellin and bikaverin biosynthesis patterns differed from those of the wild
type and paralleled the expression of key genes for both pathways, coding for geranylgeranyl pyrophosphate
(GGPP) and kaurene synthases for the former and a polyketide synthase for the latter. These results suggest
regulatory connections between carotenoid biosynthesis and nitrogen-controlled biosynthetic pathways in this
fungus. Expression of gene
ggs1
, which encodes a second GGPP synthase, was also derepressed in the
carS
mutants, suggesting the participation of Ggs1 in carotenoid biosynthesis. The
carS
mutations did not affect
genes for earlier steps of the terpenoid pathway, such as
fppS
or
hmgR
. Light induced carotenoid biosynthesis
in the wild type and
carRA
and
carB
levels in the wild-type and
carS
strains irrespective of nitrogen availability | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | American Society for Microbiology | es |
dc.relation.ispartof | Applied and Environmental Microbiology, 75 (2), 405-413. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Regulation of carotenogenesis and secondary metabolism by nitrogen in wild-type Fusarium fujikuroi and carotenoid-overproducing mutants | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Genética | es |
dc.relation.publisherversion | http://dx.doi.org/10.1128/AEM.01089-08 | |
dc.identifier.doi | 10.1128/AEM.01089-08 | |
idus.format.extent | 9 p. | es |
dc.journaltitle | Applied and Environmental Microbiology | es |
dc.publication.volumen | 75 | es |
dc.publication.issue | 2 | es |
dc.publication.initialPage | 405 | es |
dc.publication.endPage | 413 | es |
dc.identifier.idus | https://idus.us.es/xmlui/handle/11441/42223 | |