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Rational design of a bacterial transcriptional cascade for amplifying gene expression capacity

Opened Access Rational design of a bacterial transcriptional cascade for amplifying gene expression capacity

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Autor: Cebolla, Ángel
Sousa Martín, Carolina
Lorenzo, Víctor de
Departamento: Universidad de Sevilla. Departamento de Microbiología y Parasitología
Universidad de Sevilla. Departamento de Genética
Fecha: 2001
Publicado en: Nucleic Acids Research, 29 (3), 759-766.
Tipo de documento: Artículo
Resumen: Cascade regulatory circuits have been described that control numerous cell processes, and may provide models for the design of artificial circuits with novel properties. Here we describe the design of a transcriptional regulatory cascade to amplify the cell response to a given signal. We used the salicylate-responsive activators of Pseudomonas putida NahR of the naphthalene degradation plasmid NAH7 and XyIS2, a mutant regulator of the TOL plasmid for catabolism of m-xylene and their respective cognate promoters Psal and Pm. Control of the expression of xylS2 with the nahR/Psal system permitted either their selective activation with specific effectors for each protein or the simultaneous activation of both of them with salicylate. When cells face the common effector of the two regulators, both the increase in XylS2 concentration and the stimulation of its activity act synergistically on the Pm promoter, amplifying the gene expression capacity by at least one order of magnitude with res...
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Cita: Cebolla, Á., Sousa Martín, C. y Lorenzo, V.d. (2001). Rational design of a bacterial transcriptional cascade for amplifying gene expression capacity. Nucleic Acids Research, 29 (3), 759-766.
Tamaño: 225.0Kb
Formato: PDF

URI: http://hdl.handle.net/11441/41958

DOI: http://dx.doi.org/10.1093/nar/29.3.759

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