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dc.creatorCebolla, Ángeles
dc.creatorSousa Martín, Carolinaes
dc.creatorLorenzo, Víctor dees
dc.date.accessioned2016-06-07T09:29:45Z
dc.date.available2016-06-07T09:29:45Z
dc.date.issued2001
dc.identifier.citationCebolla, Á., Sousa Martín, C. y Lorenzo, V.d. (2001). Rational design of a bacterial transcriptional cascade for amplifying gene expression capacity. Nucleic Acids Research, 29 (3), 759-766.
dc.identifier.issn0305-1048es
dc.identifier.urihttp://hdl.handle.net/11441/41958
dc.description.abstractCascade regulatory circuits have been described that control numerous cell processes, and may provide models for the design of artificial circuits with novel properties. Here we describe the design of a transcriptional regulatory cascade to amplify the cell response to a given signal. We used the salicylate-responsive activators of Pseudomonas putida NahR of the naphthalene degradation plasmid NAH7 and XyIS2, a mutant regulator of the TOL plasmid for catabolism of m-xylene and their respective cognate promoters Psal and Pm. Control of the expression of xylS2 with the nahR/Psal system permitted either their selective activation with specific effectors for each protein or the simultaneous activation of both of them with salicylate. When cells face the common effector of the two regulators, both the increase in XylS2 concentration and the stimulation of its activity act synergistically on the Pm promoter, amplifying the gene expression capacity by at least one order of magnitude with respect to the individual systems. By changing the hierarchy of regulators, we showed that the specific features of the downstream regulator were crucial for the amplification effect. Directed changes in the effector profile of the regulators allowed the extension of the amplifying system to other molecular signalses
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherOxford University Presses
dc.relation.ispartofNucleic Acids Research, 29 (3), 759-766.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleRational design of a bacterial transcriptional cascade for amplifying gene expression capacityes
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Microbiología y Parasitologíaes
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Genéticaes
dc.relation.publisherversion10.1093/nar/29.3.759es
dc.identifier.doihttp://dx.doi.org/10.1093/nar/29.3.759es
idus.format.extent8 p.es
dc.journaltitleNucleic Acids Researches
dc.publication.volumen29es
dc.publication.issue3es
dc.publication.initialPage759es
dc.publication.endPage766es
dc.identifier.idushttps://idus.us.es/xmlui/handle/11441/41958

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